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Diss Factsheets
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EC number: - | CAS number: 33626-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study is done similar to OECD TG 471, GLP status unknown. The information is from a published paper.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Remarks:
- The substances have been tested in the labs where also the NTP program is carried out.
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- rel-(1S,2R,5S)-2,6,6-trimethylbicyclo[3.1.1]heptane
- Cas Number:
- 33626-25-4
- Molecular formula:
- C10H18
- IUPAC Name:
- rel-(1S,2R,5S)-2,6,6-trimethylbicyclo[3.1.1]heptane
- Test material form:
- other: liquid
- Details on test material:
- Pinane
Biocyclo [3.1.1]heptane, 2,6,6 -trimethyl-
Cas no used: 473-55-2
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: TA98, TA100, TA97 and TA1535
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Details on test system and experimental conditions:
- The selected chemicals were tested under code using a preincubation modification of the Salmonella/mammalian microsome mutagenicity (Ames) test. The tests were perfomed in two laboratories under contract to the NTP. The test chemicals for this exercise were included among other chemicals being tested for the U.S. NTP, and, therefore, were treated as if they had been slected for routine testing. The protocol used, and the data evaluation criteria have been previously described (Zeiger et al. 1992). Briefly, chemicals were tested in a preincubation procedure in strains TA98 and TA100 without metabolic activation provided by Aroclor-induced rat and hamster liver homogenates (S9). If a positive response was seen in one of these two strains, the strain/metabolic activation combination producing that response was repeated, and no further testing was performed. If no positive responses were seen, the chemical was tested in strains TA97 and TA 1535. Other Salmonella strains were occasionally used based on the response obtained with these four strains.
- Evaluation criteria:
- Some chemicals were tested more than once. In some cases this was a result of the normal practice of testing occasional duplicates under different code numbers as a quality control of the accuracy of the testing laboratories. Some chemicals yielding equivocal responses were re-tested, under a different code numbers as a quality control of the accuracy of the testing laboratory. When the results from multiple testing on single chemicals were compiled for Table 2(attached), the combination of a questionable (?) and a negative (-) response were considered (-); the combination of weakly positive (+w) and negative response was considered questionable (?). The chemicals were not decoded until testing had been completed on a sample and the data had been accepted. The chemicals tested and their CASRNs are in Appendix 1 and 2 (attached).
- Statistics:
- The statistical sampling used in this study was a two stage, double sampling scheme for estimating binomial data with misclassifications. The first stage consisted of 675 chemicals obtained via a stratified random sample of the 65,725 NAS chemicals. At the second stage, 100 chemicals were chosen by a stratified random sample of the 675.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- other:
- Positive controls validity:
- not specified
- Additional information on results:
- See Table 2-Summary Test Results (Attachment)
See Table 3-Proportions of Salmonella Mutagens in Different Datasets of Chemicals (Attachment)
Appendix 1-Test Chemicals and Salmonella Test Results (Attachement) - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Pinane was not mutagenic, according to the test results published in this article. - Executive summary:
Pinane was one of the 100 substance tested in the Ames test, according to OECD TG 471, to address the question of the proportions of mutagens among all chemical in commerce. Pinane was negative in this test.
Zeiger, E., and Margolin, B.H., 2000, The Proportions of Mutagens among Chemicals in Commerce, Regulatory Toxicology and Pharmacology 32, 219–225
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