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Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 22 - May 30, 2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

For the preparation of the highest test concentration, 100 mg of the test item was transferred
into a glass flask, which was filled up 1000 mL dilution water.
The test media was stirred vigorously for about 2 hours at room temperature. The other test
concentrations were prepared by serial dilution

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISMS:
Source:
- German Federal Environment Agency, Institut für Wasser-, Boden- und
Lufthygiene. Specimens used in the test are bred in the laboratory of the Fraunhofer IME.
- Age: juvenile Daphnia magna, 4 – 24 hours old.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

250 – 350 mg CaCO3/L

Test temperature:

18.9 and 19.2 °C

pH:

8.11 – 8.28

Dissolved oxygen:

6.30 - 9.09 mg/L

Nominal and measured concentrations:

The concentrations tested in the definitive test were selected based on results from the
range-finding tests.
Concentrations:
Control, 6.25, 12.5, 25.0, 50.0 and 100 mg test item/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers (50 mL)
- Fill volume: 50 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified drinking water.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day-night regime
- Light intensity: 765 - 802 Lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 h

TEST CONCENTRATIONS
- Range finding study: 1, 10 and 100 mg test item/L
- Test concentrations definitive test: Control, 6.25, 12.5, 25.0, 50.0 and 100 mg test item/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no

Reported statistics and error estimates:

There was no immobility of daphnids up to and including the highest test item concentration
applied. Consequently, no statistic effect evaluation could be applied.

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, the EC50 for immobility can be given as > 100 mg test item/L and the NOEC can be given as ≥ 100 mg test item/L.

Executive summary:

A study was performed to investigate the influence of the test item on the mobility of Daphnia magna according to the OECD 202.

Based on nominal concentrations, the EC50 for immobility can be given as > 100 mg test item/L and the NOEC can be given as ≥ 100 mg test item/L.

Description of key information

Not acutely toxic to aquatic invertebrates

Additional information

The acute toxicity of test item to aquatic invertebrates was investigated in a study conducted according to OECD guideline 202. Daphnia magna were exposed to nominal test substance concentrations of 6.25, 12.5, 25.0, 50.0 and 100 mg test item/L for 48 hours. The concentrations of the test item in the media were confirmed by measurements of the test item at beginning of the test and at test end using a LC-MS/MS system. 

An immobilization of the Daphnids was not observed at any tested concentrations. The EC50 is > 100 mg test item/L, the NOEC is ≥ 100 mg test item/L.

Based on the experimental results the test item is not acutely toxic to aquatic invertebrates.