Registration Dossier
Registration Dossier
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EC number: 435-080-1 | CAS number: 1070-64-0 6,8-DICHLORETHYLCAPRYLAT; ETHYL-6,8 DICHLORO OCTANOATE
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ethyl 6,8-dichlorooctanoate
- EC Number:
- 435-080-1
- EC Name:
- Ethyl 6,8-dichlorooctanoate
- Cas Number:
- 1070-64-0
- Molecular formula:
- C10H18Cl2O2
- IUPAC Name:
- ethyl 6,8-dichlorooctanoate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Batch: Ds 1099
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Remarks:
- added to water
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Taken from return sludge channels of the sewage treatment plant in Baden near Vienna (Austira)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- pH:
- 7.4 Start; 8.0 to 8.2 at the end
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- EC50 ca. 10 mg/l DCP
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The values of the EC20, EC50 and EC80 for 6,8-Dichloroethylcaprylat are >300mg/l.
- Executive summary:
The test substance did not inhibit the respiration in an activated sludge. The two highest tested concentrainns, 300 and 150 mg/l even enhanced the respiration.
The EC20, EC50 and EC80 values for 6,8-Dichloroethylcaprylate are therefore greater than 300 mg/l
No 95% confidence limits can be calculated.
The pH of the incubation mixture after the 3 hours incubation was between 8.0 and 8.2.
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