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EC number: 202-928-3 | CAS number: 101-25-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity Test Data of Existing Chemical Substances; based on the Toxicity Investigation System of the Industrial Safety and Health Law
- Author:
- JETOC (Japan Chemical Industry Ecology-Toxicology & Information Centre)
- Year:
- 1 996
- Bibliographic source:
- Chemical Substance Investigation Division, Industrial Safety and Health Department, Labour Standards Bureau, Ministry of Labor, Japan
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- Method of Ames et al. (1975), Maron and Ames (1983) and Matsushima et al. (1980)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane
- EC Number:
- 202-928-3
- EC Name:
- 3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane
- Cas Number:
- 101-25-7
- Molecular formula:
- C5H10N6O2
- IUPAC Name:
- 3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane
- Test material form:
- solid
- Details on test material:
- - Chemical name: Dinitrosopentamethylenetetramine
- CAS: 101-25-7
- EC: 202-928-3
- MW: 186.17 g/mol
- pale yellow crystalline powder
Constituent 1
- Specific details on test material used for the study:
- extra pure reagent (Tokyo Kasei Kogyo Co. Ltd), Lot AI01
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 mix (sodium phenobarbital and 5,6-benzoflavone induced)
- Test concentrations with justification for top dose:
- 0, 50, 100, 200, 500, 1000, 2000, 5000 µg/plate
- Vehicle / solvent:
- DMSO
Controlsopen allclose all
- Untreated negative controls:
- other: Solvent control served as negative control
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- with S9
- Untreated negative controls:
- other: Solvent control served as negative control
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- sodium azide
- other: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide
- Remarks:
- without S9
- Details on test system and experimental conditions:
- Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and TA1538 were obtained from Dr. B.N. Ames (University of California, Berkeley, CA, USA), and Escherichia coli WP2uvrA were from Dr. M. Ishizawa (Kyushu University, Fukuoka, Japan). To a stationary culture of the tester strains, which had been checked for the genetic markers according to Ames et al. (1975) and Maron and Ames (1983), dimethyl sulfoxide (DMSO) was added to give a final concentration of 8.2% (v/v).
The cultures were stored in aliquots of 0.2mL at -80°C. The frozen permanents were thawed and inoculated to nutrient broth (OXOID, # 2) at 1 / 500-1 / 1000 inoculation size of thawed
solution. The cultures were incubated for 10-14 hours (tester strains grew up to early stationary phase) at 37°C in a shaking water bath before starting the tests. - Evaluation criteria:
- Two-hold rule criteria was used for data evaluation (Ames et al., 1975). The chemicals are considered to be mutagenic when a dose-related increase in revertant colony count is observed and the number of revertant colonies per plate with the test substance is more than twice that of the negative control (solvent control) and when a reproducibility of test result is observed.
Mutagenic potency was calculated by following equation and maximum value of mutagenic potency was expressed as a specific acitivity on the data sheet:
mutagenic potency (induced revertants / mg test substance) = (number of induced revertants on the dose X - number of revertant on the solvent control) + mg of test chemical on the dose X.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
Any other information on results incl. tables
Toxicity (*) was observed in all tester strains at 5000 µg/plate and in TA98 (+/-), TA100 (+), TA1535 (+/-), TA1537 (+/-) and TA1538 (+/-) at 2000 µg/plate.
Number of revertants per plate, without S9
µg/plate | TA100 | TA1535 | E.coli WP2uvrA | TA98 | TA1537 | TA1538 |
0 (DMSO) | 179 | 36 | 24 | 26 | 16 | 13 |
50 | 202 | 23 | 22 | 25 | 15 | 13 |
100 | 179 | 16 | 18 | 14 | 16 | 8 |
200 | 165 | 19 | 25 | 17 | 24 | 8 |
500 | 263 | 16 | 30 | 22 | 313 | 11 |
1000 | 419 | 13 | 39 | 30 | 12 | 8 |
2000 | 0 * | 0 * | 32 | 0 * | 0 * | 0 * |
5000 | 0 * | 0 * | 0 * | 0 * | 0 * | 0 * |
pos. control | 1068 | 269 | 375 | 277 | 234 | 297 |
Number of revertants per plate, with S9
µg/plate | TA100 | TA1535 | E.coli WP2uvrA | TA98 | TA1537 | TA1538 |
0 (DMSO) | 199 | 12 | 26 | 39 | 24 | 18 |
50 | 183 | 11 | 21 | 25 | 22 | 24 |
100 | 222 | 9 | 22 | 31 | 22 | 14 |
200 | 243 | 11 | 24 | 31 | 19 | 18 |
500 | 452 | 19 | 40 | 34 | 20 | 22 |
1000 | 825 | 15 | 51 | 61 | 12 | 13 |
2000 | 588 | 7 * | 57 | 62 * | 3 * | 6 * |
5000 | 0 * | 0 * | 18 * | 0 * | 0 * | 0 * |
pos. control | 501 | 203 | 646 | 288 | 305 | 252 |
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this test, the test item was positive causing gene mutations by base pair changes in the genome of the tester strain S. typhimurium TA100 with and without metabolic activation.
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