N,N'-[(methylimino)dipropane-1,3-diyl]dioleamide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to attached document

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

> 0.108 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr. (dissolved fraction)

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.108 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr. (dissolved fraction)

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 0.108 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr. (dissolved fraction)

Basis for effect:

mortality

Conclusions:

N, N’-[(methylimino)bis (trimethylene)]bis(oleamide) caused no effect to Daphnia magna up to the limit of solubility based on information from the related read-across substance N,N’[(methylimino)bis(trimethylene)]bis(stearamide). 

Description of key information

N, N’-[(methylimino)bis (trimethylene)]bis(oleamide) caused no effect to Daphnia magna up to the limit of solubility based on information from the related read-across substance N,N’[(methylimino)bis(trimethylene)]bis(stearamide). 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.108 mg/L

Additional information

Available information from an OECD 202 guideline study with the test substance N,N’[(methylimino)bis(trimethylene)]bis(stearamide) were used to evaluate short-term toxicity to aquatic invertebrates of N, N’-[(methylimino)bis(trimethylene)]bis(oleamide).

In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test itemN,N’‑[(methylimino)bis(trimethylene)]bis(stearamide) were determined according to OECD 202 (2004).

The test item is a yellow solid with a low water solubility of 10 - 500 µg/L.In the definitive test the solventTetrahydrofuran containing 0.1% formic acid (THF+FA) was used as it helps dissolving the test item in the test medium at the limit of the water solubility. As observed in the range finding test, the test item is insoluble / instable in the test medium and under test conditions and, therefore, the use of a solvent was necessary to prepare a defined test item concentration at the limit of the water solubility of the test item. A limit concentration of 0.100 mg/L of the test item was tested.

The study was performed over a period of 48 hours under semi-static conditions with a renewal of test solution after 24 hours as a worst case exposure. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to the limit concentration, the solvent control and the control.

The limit concentrationwas colorless and visually clear throughout the exposure period.

The concentration of the test item N,N’-[(methylimino)bis(trimethylene)]bis(stearamide) was analytically verified via LC-MS/MS in the fresh media at the start of the exposure (0 hours and 24 hours) and in the corresponding old media and the renewal of the test solutions and at the end of the exposure (24 hours and 48 hours) in the limit concentration, the solvent control and the control.

The measured concentration of the test item in the fresh media (0 and 24 hours) was 64% and 306% of the nominal test item concentration and 101% and 69 % in the corresponding old media (24 and 48 hours). The geometric mean measured concentration is 0.108 mg/L. This concentration is considered to be the maximum solubility level of the test item in test medium under test conditions..

The effect levels given are based on the geometric mean measured concentration of the test item N,N’-[(methylimino)bis(trimethylene)]bis(stearamide):

EC₁₀/ EC₅₀/ EC₁₀₀24 hours: > 0.108 mg/L

EC₁₀/ EC₅₀/ EC₁₀₀48 hours: > 0.108 mg/L

The validity criteria of the test guidelines were fulfilled.

 

The test item N,N’‑[(methylimino)bis(trimethylene)]bis(stearamide) caused no effect to Daphnia magna up to the limit of solubility in the test medium and under the test conditions.