Registration Dossier
Registration Dossier
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EC number: 285-249-5 | CAS number: 85049-76-9
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
EC50 (7d) > 63.9 mg/l as measured value, equivalent to a nominal concentration of 100 mg/l.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater plants:
- 7.6 mg/L
Additional information
No data on Acid Green 104 was available, thus a read-across approach was adopted. In particular, available data on Similar Substance 01, i.e. cobalt complex with 2 phenyl-azo-naphtyl ligands functionalised by similar groups, was used. A detailed description of the read-across approach is attached in section 13.
The toxicity of Similar Substance 01 was assessed in a study conducted according to OECD guideline 221, due to nature of test substance, i.e. a dye with intensive coloration possibly interfering with the growth of unicellular algae used in a regular OECD 201 test.
A stock solution at 100 mg/l was prepared. This stock was used to prepare the final test concentrations of 1.56, 3.12, 6.25, 12.5, 25, 50 and 100 mg/l. The test was conducted as static test. After an exposure time of 7 days effect levels for growth rate based on frond number and dry weight were determined:
- frond number: EC50 > 63.9 mg/l, EC10 = 7.6 mg/l
- dry weight: EC50 > 63.9 mg/l, EC10 = 8.7 mg/
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