Hydrogen bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

unknow

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Lithium sodium (4-((5-chloro-2-hydroxyphenyl)azo)-2,4-dihydro-5-methyl-3H-pyrazol-3-onato(2-))(3-((4,5-dihydro-3-methyl-1-(4-methylphenyl)-5-oxo-1H-pyrazol-4-yl)azo)-4-hydroxy-5-nitrobenzenesulfonato(3-)) chromate(2-)

EC Number:

414-250-7

EC Name:

Lithium sodium (4-((5-chloro-2-hydroxyphenyl)azo)-2,4-dihydro-5-methyl-3H-pyrazol-3-onato(2-))(3-((4,5-dihydro-3-methyl-1-(4-methylphenyl)-5-oxo-1H-pyrazol-4-yl)azo)-4-hydroxy-5-nitrobenzenesulfonato(3-)) chromate(2-)

Cas Number:

149564-66-9

Molecular formula:

C27H19ClCrLiN9NaO9S

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: unknown
- Stability under storage conditions: unknown
- Stability under test conditions: unknown
- Solubility and stability of the test substance in the solvent/dispersant/vehicle/test medium: the vehicle used is water – the solubility of the test material in water is >81 g/L at 26°C and pH 7
- Reactivity of the test substance with the solvent/vehicle /test medium (if applicable): unknown
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: dilution in water
- Preliminary purification step (if any): unknown
- Preparation of a nanomaterial dispersion (incl. dilution): unknown
- Final dilution of a dissolved solid, stock liquid or gel: unknown
- Final preparation of a solid: unknown

Test animals

Species:

rabbit

Strain:

New Zealand White

Remarks:

Albino

Test system

Type of coverage:

semiocclusive

Preparation of test site:

not specified

Vehicle:

water

Controls:

not specified

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 500 mg

Duration of treatment / exposure:

4 hours

Observation period:

Not specified

Number of animals:

3 Animals

Details on study design:

OBSERVATION TIME POINTS
(indicate if minutes, hours or days)
- Erythema Score: overall at 24-48 and 72 hours
- Edema Score: overall at 24-48 and 72 hours
SCORING SYSTEM:
- Method of calculation: Draize calculation method.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

edema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

1

Reversibility:

not specified

Remarks on result:

other: Max. duration: 24h; Max. value at the end of observation: 0 (related to all animals)

Results 2

Irritation parameter:

edema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

1

Reversibility:

not specified

Remarks on result:

other: Max. duration: 24h; Max. value at the end of observation: 0 (related to all animals)

Results 3

Irritation parameter:

edema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0

Max. score:

1

Reversibility:

not specified

Remarks on result:

other: Max. duration: 24h; Max. value at the end of observation: 0 (related to all animals)

Irritant / corrosive response data:

Reversibility of any observed effect: Changes fully reversible within 1 days

Any other information on results incl. tables

Results:
- Erythema: due to the red discoloration of the skin a determination was not possible.
- Oedema: max. duration: < 24 h

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

not irritant