Anilinoacetonitrile

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards. No data on purity of test substance. No GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Principles of method if other than guideline:

The test substance was applied to the conjunctival sac of white Vienna rabbits. The non-treated adjacent eye served as control, to which talcum powder was applied. The animals were observed several times on the treatment day and 8 days afterwards, and findings were recorded on working days. The test substance was not washed out 24 hours after instillation. Findings were graded using a scheme convertable into the Draize scheme.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Anilinoacetonitrile

EC Number:

221-129-0

EC Name:

Anilinoacetonitrile

Cas Number:

3009-97-0

Molecular formula:

C8H8N2

IUPAC Name:

2-(phenylamino)acetonitrile

Details on test material:

- Name of test material (as cited in study report): Phenylglycinnitril
- Physical state: solid
- Analytical purity: no data

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

50 mm3

Duration of treatment / exposure:

continuous

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 24, 48 h

Score:

1

Reversibility:

not reversible

Remarks on result:

other: Individual Scores of the BASF Test have been converted into the OECD Draize Scheme.

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 24, 48 h

Score:

0

Remarks on result:

other: Individual Scores of the BASF Test have been converted into the OECD Draize Scheme.

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 24, 48 h

Score:

1

Reversibility:

fully reversible within: 8 days

Remarks on result:

other: Individual Scores of the BASF Test have been converted into the OECD Draize Scheme.

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 24, 48 h

Score:

0

Remarks on result:

other: Individual Scores of the BASF Test have been converted into the OECD Draize Scheme.

Any other information on results incl. tables

Table1: Individual Scores of cornea, iris and conjunctivae that have been converted from BASF test into the OECD Draize Scheme.

	time	animal 1	animal 2
corneal opacity	24 h	1	1
	48 h	1	1
	72 h	no data	no data
	8 d	1	1
iris	24 h	0	0
	48 h	0	0
	72 h	no data	no data
	8 d	0	0
conjunctiva erythema/edema	24 h	1/0	1/0
	48 h	1/0	1/0
	72 h	no data	no data
	8 d	0/0	0/0