Registration Dossier
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EC number: 262-134-8 | CAS number: 60270-33-9
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
72 hr ErC50 = 1.73 mg/L, 72 hr NOEC (growth rate) = 0.36 mg/L
72 hr EyC50 = 0.72 mg/L, 72 hr NOEC (biomass) = 0.36 mg/L
(Scenedesmus subspicatus, OECD guideline 201, static, GLP, RL1)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.73
- EC10 or NOEC for freshwater algae:
- 0.36
Additional information
In a 72 hours toxicity study, the cultures of Scenedesmus subspicatus were exposed to DIMAPDO lactate at nominal concentrations of 1.0, 2.0, 4.0, 8.0 and 16.0 mg/L under static conditions in accordance with the OECD guideline 201 (1984) andEU Method C.3 (Algal Inhibition test; Commission Directive 92/69/EEC). The % growth inhibition in the treated algal culture as compared to the control ranged from 2% to 107%.
The NOEC and EC50 values based on % growth inhibition were 1.0 and 2.7 mg/L, respectively. Based on the geometric mean measured test concentrations of the centrifuged test media the EC50 value was 1.6 mg/L and the NOEC was 0.40 mg/L. Because there was adiscontinuity in measured concentration for nominal concentration 2 mg/Lthese values were not used for further risk assessment. Based on the geometric mean measured test concentrations of the untreated test media the EC50 was 2.1 mg/L and the NOEC was 0.44 mg/L.
The study was conducted with the lactic acid salt of DIMAPDO. Therefore the EC50 and NOEC values of DIMAPDO based on % growth inhibition and the test concentrations of untreated media were calculated as 1.73 mg/L and 0.36 mg/L active ingredient.
The following abnormalities were noted: After 72 hours there were no abnormalities detected in the control or test cultures at 1.0 mg/L, however some enlarged cells were observed to be present in the test cultures at 2.0, 4.0, 8.0 and 16.0 mg/L. The test material was observed to form a homogenous dispersion in culture medium.
This toxicity study is classified as acceptable and satisfies the guideline requirements for a OECD guideline 201 Scenedesmus subspicatus toxicity study.
Results Synopsis
Test Organism: Scenedesmus subspicatus
Test Type: Static
72 hr EC50: 1.73 mg a.i./L 95% C.I.: 1.49 to 1.98 mg a.i./L
72 hr NOEC: 0.36 mg a.i./L
Endpoint(s) Effected: growth inhibition
72 hr EC50: 0.72 mg a.i./L 95% C.I.: 0.59 - 0.91mg a.i./L
72 hr NOEC: 0.36 mg a.i./L
Endpoint(s) Effected: biomass
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