Mycophenolic acid

Vapour pressure

Administrative data

Endpoint:

vapour pressure

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 26, 1992 to July 1, 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The protocol follows in part the recommendations made in the published literature and in the Environmental Assessment Technical Assistance Handbook (Food and Drug Administration, March 1987).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: The protocol follows in part the recommendations made in the published literature and in the Environmental Assessment Technical Assistance Handbook (Food and Drug Administration, March 1987).

Principles of method if other than guideline:

A known amount of test compound is volatilized under a nitrogen stream. An estimated vapor pressure is determined by comparison of the percent compound loss per day to a reference standard of known vapor pressure volatilized under similiar conditions.

GLP compliance:

not specified

Type of method:

effusion method: by loss of weight or by trapping vaporisate

Test material

Test material information

Constituent 1

Reference substance name:

Mycophenolic acid

EC Number:

246-119-3

EC Name:

Mycophenolic acid

Cas Number:

24280-93-1

Molecular formula:

C17H20O6

IUPAC Name:

6-(4-hydroxy-6-methoxy-7-methyl-3-oxo-1,3-dihydro-2-benzofuran-5-yl)-4-methylhex-4-enoic acid

Details on test material:

Molecular formula: C17H20O6
CAS registry number: 24280-93-1
p K: 4.5
Solubility, mg/mL - pH 5: 0.03
pH 7: 4.0

Results and discussion

Vapour pressure

Temp.:

22 °C

Vapour pressure:

0 Torr

Remarks on result:

other: The vapor pressure of mycophenolic acid was estimated to be 3.2 x 10-7 Torr @ 22.0 degree C.

Any other information on results incl. tables

The volatility of mycophenolic acid was estimated relative to 4,4' DDT. The experiment was conducted over a 5 day period. The loss per day for volatilization of mycophenolic acid and the 4,4' DDT allowed for calculation of acid vapor pressure.

Mycophenolic acid had a measureable vapor pressure at 22°C. The vapor pressure of mycophenolic acid was estimated to be 3.2 x 10 -7 Torr.

Applicant's summary and conclusion

Conclusions:

The vapor pressure of mycophenolic acid was estimated to be 3.2 x 10-7 Torr @ 22.0 degree C.

Executive summary:

Purpose.

The objective of this experiment was to determine the estimated vapor pressure of mycophenolic acid.

Performance.

A compound is examined for volatility by estimation of its vapor pressure. A known amount of test compound is volatilized under a nitrogen stream. An estimated vapor pressure is determined by comparison of the percent compound loss per day to a reference standard of known vapor pressure volatilized under similiar conditions. The reference standard used in this experiment was 4,4' DDT. n-nonadecane was used as the internal standard for the 4,4' DDT. Kimble 20 mL glass scintillation vials containing 1 mL of 0.1024 mg/mL 4,4' DDT in methanol were volatilized under a nitrogen stream. When four of the reaction vials approached dryness, they were removed from the nitrogen stream, labeled as initial reference samples and taken to dryness at ambient conditions. They were capped when dry. The other four reaction vials remained under the nitrogen stream for five days before removal and being capped. The 4,4' DDT reference standards were redissolved in hexane containing the internal standard n-nonadecane. The reference standards were analyzed by GC/FID along with a 4,4' DDT standards calibration set. The test sample, mycophenolic acid was subjected to the same conditions as the 4,4' DDT reference standard. Analysis proceeded by HPLC without the use of an internal standard.

Results.

The vapor pressure of mycophenolic acid was estimated to be 3.2 x 10 -7Torr @ 22.0 degree C.