Phenol, dodecyl-, branched

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-04-19 until 1995-04-21

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phenol, dodecyl-, branched

EC Number:

310-154-3

EC Name:

Phenol, dodecyl-, branched

Cas Number:

121158-58-5

Molecular formula:

C18H30O

IUPAC Name:

4-(3,4,5,6-tetramethyloctan-2-yl)phenol

Constituent 2

Reference substance name:

Dodecylphenol

EC Number:

248-312-8

EC Name:

Dodecylphenol

Cas Number:

27193-86-8

IUPAC Name:

2-dodecylphenol

Details on test material:

- Name of test material (as cited in study report): Dodecylphenol T
- Substance type: technical product
- Physical state: liquid
- Analytical purity: 100%
- Impurities (identity and concentrations): not mentioned
- Composition of test material, percentage of components: not mentioned
- Purity test date: not mentioned
- Lot/batch No.: continuously production; 3630/81325
- Date of production: 1991-04-25
- Stability under test conditions: 1 year
- Storage condition of test material: not mentioned

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

- Dispersion: no
- Vehicle, solvent: acetone
- Concentration of stock solution: 1 g/l

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus, IRCHA
- Source: laboratory breed
- Age at study initiation: not older than 24 hours
- Method of breeding: The females were maintained in M4 medium in 1 l beakers. A reddish dark-brown coloration of the animals indicated that they were healthy. At two to three day intervals, the exuviae were siphoned off and the water was changed. In the course of this, the offspring was removed from the breeding vessels. At regular intervals of 4-5 weeks, offspring was isolated from the vessels. They served as a basis for further breeding.
- Feeding during test: none

ACCLIMATION
- Acclimation period: Females were seperated from the offspring 24 hours before the start of the test. The offspring produced overnight were transferred into test water and were used for the test.
- Type and amount of food: Scenedesmus subspicatus, the amount of food added is such that it is consumed by the animals
- Feeding frequency: daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

not mentioned

Test temperature:

20 °C ± 1 °C

pH:

7.4 - 7.5

Dissolved oxygen:

7.8 - 8.1 mg/l

Salinity:

CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 7 H2O: 123 mg/l; NaHCO3: 63 mg/l; KCl: 5.5 mg/l
Ca/Mg ratio: 4:1
Na/K ratio: 10:1

Nominal and measured concentrations:

nominal concentration: 0 (control) / 20 / 28 / 40 / 56 / 80 / 112 / 160 / 220 / 320 / 450 µg/l and acetone 450 µl/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Round-bottom test tubes
- Material, size, headspace, fill volume: glass, calibrated to 10 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 7 H2O: 123 mg/l; NaHCO3: 63 mg/l; KCl: 5.5 mg/l)
- Ca/Mg ratio: 4:1
- Na/K ratio: 10:1
- Conductivity: not mentioned
- Culture medium different from test medium: yes, M4 medium, Elendt
- Intervals of water quality measurement: not mentioned

OTHER TEST CONDITIONS
- Adjustment of pH: not adjusted
- Photoperiod: not applicable
- Light intensity: no light during test

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation after 24 and 48 hours; O2 concentration and pH 0 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned
- Range finding study: not mentioned

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

106 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

92.7 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Remarks on result:

other: 95% CL: 81.1 - 105.9 µg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

56 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

160 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Concentrations: 0.9 and 1.9 mg/l potassium dichromate
- Results: 50% at 0.9 mg/l and 100% at 1.9 mg/l immobilised daphnia after 24 h

Reported statistics and error estimates:

The EC50 (48h) value was calculated with Propit-analysis according to Cavalli-Sforza (1972), the EC50 (24h) was calculated graphically (dose-response relationship).

Any other information on results incl. tables

Table #1: Effect of o-tert.-Butylphenol on immobilisation of Daphnia magna

concentration[µg/L]	24 hour values			48 hour values
	number		%	number		%
	mobile	immobile	immobile	mobile	immobile	immobile
0 (control)	20	0	0	20	0	0
20	20	0	0	20	0	0
28	20	0	0	20	0	0
40	20	0	0	20	0	0
56	20	0	0	19	1	5
80	16	4	20	15	5	25
112	7	13	65	5	15	75
160 *	0	19	100	0	19	100
220	0	20	100	0	20	100
320	0	20	100	0	20	100
450	0	20	100	0	20	100
acetone	20	0	100	20	0	0

* 19 daphnia deployed

Applicant's summary and conclusion

Validity criteria fulfilled:

yes