Registration Dossier
Registration Dossier
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EC number: 203-896-3 | CAS number: 111-69-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1975 to 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Followed appropriate guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- Adiponitrile
- EC Number:
- 203-896-3
- EC Name:
- Adiponitrile
- Cas Number:
- 111-69-3
- Molecular formula:
- C6H8N2
- IUPAC Name:
- hexanedinitrile
- Details on test material:
- - Name of test material (as cited in study report): Adiponitrile
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat-liver homogenate activation system
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- 100% ethanol
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- 2-Nitrofluorene 25ug and N-methyl-N'-nitro-N-nitrosoguanidine 2 ug were also used as postive controls
Migrated to IUCLID6: 50 ug
Results and discussion
Test results
- Species / strain:
- other: TA 1535, TA 1537, TA 1538 and TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- compound was not mutagenic in the microbial assays either in the presence or absence of a liver microsomal system
- Cytotoxicity / choice of top concentrations:
- not specified
- Remarks:
- compound was not mutagenic in the microbial assays either in the presence or absence of a liver microsomal system
- Additional information on results:
- NOTE: TA 100 was specified in protocol, results were not reported.
Applicant's summary and conclusion
- Conclusions:
- Adiponitrile was not mutagenic in the microbial assays either in the presence or absence of a liver microsomal activation system.
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