Methanesulphonyl chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2007-06-07 to 2009-06-22

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: 1a: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Methanesulphonyl chloride

EC Number:

204-706-1

EC Name:

Methanesulphonyl chloride

Cas Number:

124-63-0

Molecular formula:

CH3ClO2S

IUPAC Name:

methanesulfonyl chloride

Details on test material:

- Name of test material: Methanesulfonyl chloride
- Lot/batch No.: MSC B14GPD/Arkema
- Purity: > 99.9%
- Date of receipt: 2007-03-15
- Appearance: colourless or yellow liquid
- Water solubility: 1100 mg/L at 20 °C
- Density at 20°C: 1478 kg/m3
- Dissolved in water

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not relevant

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sample storage before analysis: no storage
- Samples treatment: direct analysis

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION
A stock solution of the test item diluted in water was prepared one day (22 hours) before the beginning of the test (time 0) by mixing 100 mg (67.7µL) of MSC in 1 L of dilution water for preliminary and definitive test.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus (Cladocera, Crustacea)
- Strain: clone 5 or clone A
- Source: come from a stock breading in the laboratory reared in VOLVIC® water added with vitamin B12, Na2SeO3, 5H2O, spiruline, Calcium, Magnesium unicellular green fresh-water algae Pseudokirchneriella subcapitata and Chlorella Vulgaris.
- Age at study initiation: less than 24 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

no data

Test temperature:

remained between 18 and 22 °C

pH:

At the beginning of the test, from 7.24 to 4.68 (concentration 50 mg/L).
At the end of the test, from 7.20 to 5.05 (concentration 50 mg/L).
pH is considerably lowered for the higher nominal concentrations of the test item due to the hydrolysis of the substance into MSA.

Dissolved oxygen:

from 8.2 to 9.1 mg/L O2

Salinity:

no data

Nominal and measured concentrations:

Nominal concentrations: 10 - 13.8 - 19 - 26.3 - 36.2 and 50 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 30 mL glass bottles stoppered with PTFE bungs and sealed with aluminium caps
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Renewal: no

TEST MEDIUM / WATER PARAMETERS
- Ultrapure water used for the preparation of dilution water (resistivity > 18MΩ).

OTHER TEST CONDITIONS
- test flasks incubated in darkness

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.38

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

34 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

45 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

EC50 values determined at 24 and 48h post exposure based on nominal concentrations for Methanesulfonic Acid (MSA):
- EC50-24h = 38 mg/L
- EC50-48h = 29 mg/L

Results with reference substance (positive control):

EC50 obtained on 31/05/2007 was 0.95 mg/L.

Reported statistics and error estimates:

The inhibition of mobility data are analyzed using an Excel program. It was designed to calculate the EC50 value and the 95% confidence interval. Probit analysis is generally used to calculate the 24 and 48-hour EC50 values.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

See conclusions

Conclusions:

Quality criteria:
- The immobilisation in the control should not exceed 10 per cent at the end of the test;
- Daphnids in the control have not be trapped at the surface of the water;
- The dissolved oxygen concentration remained above 3 mg/L over the test period.

Executive summary:

Acute toxicity of Methanesulfonyl chloride to Daphnia magna has been investigated through OECD Guideline 202.

Daphnia magna were exposed for 48 hours to an aqueous solution of Methanesulfonyl chloride at different nominal concentrations of 10 - 13.8 - 19 - 26.3 - 36.2 and 50 mg/L.

EC 50 - 48h determined is of 34 mg/L and EC50 - 24h is of 45 mg/L. As the substance is known to hydrolyze quite easily into methanesulfonic acid (MSA), EC50 -48h and EC50 -24h were calculated for MSA, and were respectively of 29 mg/L and 38 mg/L.

The quality criteria were fulfilled.