Methyl tetrahydro-2-furancarboxylate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 24 April 1996 to 26 April 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according to international guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl tetrahydro-2-furancarboxylate

EC Number:

420-670-1

EC Name:

Methyl tetrahydro-2-furancarboxylate

Cas Number:

37443-42-8

Molecular formula:

C6H10O3

IUPAC Name:

methyl oxolane-3-carboxylate

Test material form:

other: liquid

Details on test material:

Identity: Methfat
Chemical name: Methyl tetrahydro-2-furoate
Batch number: 17-5C03 (GLFC No.002)
Expiry: 7 November 1996
Purity: 99.4%
Appearance: Clear liquid
Storage conditions: In darkness at room temperature
Date received: 17 November 1995

Test solutions

Details on test solutions:

Method of preparation
Elendt M7 medium was prepared using analytical grade reagents and reverse osmosis purified water.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Culture:
Brood stocks of Daphnia magna were cultured under a 16 h light : 8 h dark photoperiod at 20°C in polypropylene vessels containing two litres of Elendt M7 medium. Cultures were fed daily with a suspension of mixed algae (predominantly Scenedesmus and Selenastrum spp). Culture conditions ensure that reproduction is by partbenogenesis.

Gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

19+/-1°C

pH:

From 7.5 to 7.9

Dissolved oxygen:

From 7.8 to 8.4 mg O2/l

Nominal and measured concentrations:

Nominal concentrations: 2.2. 4.6. 10. 22. 46. 100 and 220 mg/l
Mean measured concentrations: 1.7. 3.7. 8.5. 19. 42, 93 and 200 mg/l

Details on test conditions:

Experimental design
Seven test concentrations plus one control each in duplicate (20 animals per test group).
Ten first instar Daphnia were placed at random in each glass jar containing 200 ml of prepared test medium, or diluent medium only, as appropriate, to give a loading of 20 ml test solution per organism. The jars were sealed with screw-down lids to minimise evaporation losses.

Environmental conditions
Cultures were maintained at 19 ± I °C under a photoperiod of 16 hours light : 8 hours dark and without supplementary aeration or feeding during the 48 hour exposure period.
The temperature in each vessel was measured daily and the pH and dissolved oxygen levels recorded at the start and at the end of the study.
Criterion of effect
Daphnia were considered to be immobilised if they were unable to swim after approximately 15 seconds of gentle agitation.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

There was no incidence of significant immobilisation recorded in any of the test or control vessels during this study (see Table I). All results are expressed in terms of mean measured concentration, which for the highest concentration tested was 93% of nominal.
The measured concentration concentration at the highest test level (220 mg/l) ranged from 99% of nominal at 0 hours to 87% of nominal at 48 hours. demonstrating that near nominal concentrations were achieved and maintained during this study.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48h EC50 (immobilisation) value for Methfat with Daphnia magna is > 200 mg/l.