Methyl tetrahydro-2-furancarboxylate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From march 21 to april 18, 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP, guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl tetrahydro-2-furancarboxylate

EC Number:

420-670-1

EC Name:

Methyl tetrahydro-2-furancarboxylate

Cas Number:

37443-42-8

Molecular formula:

C6H10O3

IUPAC Name:

methyl oxolane-3-carboxylate

Details on test material:

Identity: Methfat
Chemical name: Methyl tetrahydro-2-furoate
Batch number: 17-5C03 (GLFC No.002)
Expiry: 7 November 1996
Purity: 99.4%
Appearance: Clear liquid
Storage conditions: In darkness at room temperature
Date received: 17 November 1995

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Preparation:
The test and standard substances (sodium benzoate) were dispersed directly in nutrient medium without the aid of auxiliary solvents or dispersing agents to give the following series:
(a) Inoculated nutrient medium.
(b) 3 mg/l test substance.
(c) 3 mg/l sodium benzoate.
(d) mg/l test substance plus 3 mg/l sodium benzoate.

Inoculation of the stock nutrient medium with activated sewage sludge filtrate (at the rate of 1 ml of inoculum per litre) was performed immediately prior to the preparation of series (a), (b), (c), and (d).

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

74

Sampling time:

28 d

Details on results:

The "pass" level of 60% was reached within 10 days of achieving the 10% level and, consequently, the test substance may be termed as readily biodegradable.
Cultures containing both test and standard substances combined showed an oxygen depletion value 2% higher than that anticipated on the basis of results from separate cultures at Day 14. Consequently Methfat is not considered to have had an inhibitory effect on sewage bacteria under the conditions of this test.

Oxygen depletions in the inoculated control series were within the prescribed limits (inoculated control: ≤ 1.5 mg02/l after 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test substance may be termed as readily biodegradable.