Dimethyl 2-aminoterephthalate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1995

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl 2-aminoterephthalate

EC Number:

226-364-2

EC Name:

Dimethyl 2-aminoterephthalate

Cas Number:

5372-81-6

Molecular formula:

C10H11NO4

IUPAC Name:

1,4-dimethyl 2-aminobenzene-1,4-dicarboxylate

Constituent 2

Reference substance name:

dimethyl-2-aminoterephthalate

IUPAC Name:

dimethyl-2-aminoterephthalate

Details on test material:

purity > 99.9 % (m/m)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no stock solution was made because of low water solubility. The product was weighted directly into the testing vessel.
concentrations: 250 and 1000 mg/l. The test concentrations were treated for 15 min with ultrasonic. Afterwards the pH was adapted with 0.1 mol/l NaOH.
The incubation with test item was done before measurement of breathing activity at 19.7 - 20.8 °C over 3 hours. The composition of the reaction media was equivalent to EECDirective. The pH-value of the culture solution was 7.0 and 7.1 before the measurement.

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

no data

Test solutions

Vehicle:

no

Details on test solutions:

vehicle = water
concentrations: 250 and 1000 mg/l. The test concentrations were treated for 15 min with ultrasonic. Afterwards the pH was adapted with 0.1 mol/l NaOH.
The incubation with test item was done before measurement of breathing activity at 19.7 - 20.8 °C over 3 hours. The composition of the reaction media was equivalent to EECDirective. The pH-value of the culture solution was 7.0 and 7.1 before the measurement.

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Activated sludge out of municipal water treatment plant was used as inoculum.
Source: Frankfurt-Sindlingen
Pre-treatment: according to EG guideline
Inoculum was neither acclimatized to the test item nor to the reference substance.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Post exposure observation period:

no data

Test conditions

Hardness:

no data

Test temperature:

19,7 - 20,8 °C

pH:

7,0 - 7,1

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

2 groups each nominal: 250, 500 and 1000 mg/l test item
3 control groups with no treatment
1 control group of 5, 20 and 30 mg/l 3,5-Dichlorophenol

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

Up to the highes testconcentration there was no inhibition > 20%. Therefore all EC values are above 1000mg/l.
effectiv concentration:
EC 20 = >1000 mg/l
EC50 = > 1000 mg/l
EC80 = >1000 mg/l

Results with reference substance (positive control):

EC50 of 3,5-Dichlorophenol was tested between 5 and 30 mg/l. The EC50 is > 1000 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

reliable source

Conclusions:

Up to 1000 mg/l test item the inhibition was not above 20 %. EC 50 > 1000 mg/l.

Executive summary:

Up to 1000 mg/l test item the inhibition was not above 20 %. EC 50 > 1000 mg/l.