2-(ethylnitroamino)ethyl nitrate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 October 2019 - 25 October 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Test method according to EEC method A.6. and OECD Guideline 105. GLP study.

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Key result

Water solubility:

4.31 g/L

Conc. based on:

test mat.

Incubation duration:

72 h

Temp.:

20 °C

pH:

>= 3.29 - <= 3.67

Details on results:

Solubility of the test item at 20 ± 0.5ºC and at pH ranging 3.29–3.67 in water is 4.31 g/L.

Table 1. Results of the preliminary test

0.1g in x ml of solution	0.1	0.5	1	2	10	100	> 100
Soluble (+/-)	-	-	-	-	-	+	+
Approximate solubility, g/l	> 1000	1000 to 200	200 to 100	100 to 50	50 to 10	10 to 1	<1

Table 2. Study solution in water

Sample day/ flask		Weight of the sample [mg]	Dilution [mL]	Cp [mg/mL]	Peak area		Average peak area Ap	X [g/kg]
1	1	200.72	10-1-10	2.0072	428739	428744	428741.5	4.3810
	2	200.79		2.0079	427855	427935	427895	4.3708
2	3	202.41	10-1-10	2.0241	418418	417766	418092	4.2676
	4	202.08		2.0208	418209	417335	417772	4.2725
3	5	201.15	10-1-10	2.0115	420135	419586	419860.5	4.3172
	6	201.20		2.0120	413070	414290	413680	4.2436

Table 3. Results of  Et-NENA (Ethyl-2-nitratoethylnitramine)solubility in water

Mixing time at 30 ºC [days, flasks]	1		2		3
Samples	1	2	3	4	5	6
Temperature, ºC	19.9		19.5		19.5
pH	3.67		3.38		3.29
Concentration of Et-NENA (Ethyl-2-nitratoethylnitramine), g/kg	4.38	4.37	4.27	4.27	4.32	4.24
Average concentration of Et-NENA (Ethyl-2-nitratoethylnitramine), g/kg	4.38		4.27		4.28
Average concentration of Et-NENA (Ethyl-2-nitratoethylnitramine), g/kg	4.31

The solubility of Et-NENA (Ethyl-2-nitratoethylnitramine) in water is 4.31 g/kg. The density of this solution is about 1 kg/l (about water density). After recalculation:

The solubility of Et-NENA (Ethyl-2-nitratoethylnitramine) at pH 3.29 – 3.67 in water is 4.31 g/l.

Conclusions:

Solubility of Et-NENA at 20 ± 0.5ºC and at pH ranging 3.29–3.67 in water is 4.31 g/L.

Executive summary:

The water solubility test of Et-NENA was performed in accordance with EEC method A.6 and OECD 105. About five times the quantity of test item determined in the preliminary test was weighed into each of three glass vessels at 100 ml fitted with glass stoppers. 70 ml of double distilled water was added to each vessel. These closed vessels were agitated in an incubator at 30 ºC with magnetic stirrers. After one day, one of the flask was equilibrated for 24 h at the test temperature 20 ± 0.5 ºC with occasional shaking. The other two flasks were treated similarly after initial equilibration at 30 ºC for two and three days respectively. Two replicates were performed. The samples taken during saturation were then separated using a vial-centrifuge to give the clear liquid phase and the concentration of the test substance in the clear solvent phase was determined by a validated HPLC method. The solubility of Et-NENA in distilled water at pH 3.29 -3.67 was determined to be 4.31 g/l at 20 ± 0.5 °C.

Description of key information

Key study: Test method EEC A.6 and OECD 105. GLP study. The solubility of Et-NENA in distilled water at pH 3.29 -3.67 was determined to be 4.31 g/l at 20 ± 0.5 °C.

Key value for chemical safety assessment

Water solubility:

4.31 g/L

at the temperature of:

20 °C