6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid, compound with 2,2',2''-nitrilotriethanol (1:3)

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-02-09 to 1995-05-07

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP study

Qualifier:

according to guideline

Guideline:

EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Buffers:

- pH 4: standard acetate buffer pH 4.65 (0.1 mol/l acetic acid -f 0.1 mol/l sodium acetate) -H 15 ml glacial acetic acid per liter.
- pH 7: standard phosphate buffer ( 0.041 mol/l di-sodiumhydrogenphosphate -i- 0.028 mol/l potassium-dihydrogenphosphate).
- pH 9: standard borate buffer (0.043 mol/l di-sodium-tetraborate + 0.017 mol/l potassium-dihydrogenphosphate).
The solubility depends on pH value. The solubility of the test substance in the buffer solution pH 4 at 20°C is < 0.002 g/l. This value is close to the detection limit (0.001 g/l) of our anaytical method. Therefore, hydrolysis test at pH 4 was not performed.

Duration:

5 d

pH:

7

Temp.:

50

Initial conc. measured:

0.302 g/L

Duration:

2.4 h

pH:

7

Temp.:

50

Initial conc. measured:

0.302 g/L

Duration:

5 d

pH:

9

Temp.:

50

Initial conc. measured:

0.304 g/L

Duration:

2.4 h

pH:

9

Temp.:

50

Initial conc. measured:

0.304 g/L

Number of replicates:

two replicates for each pH value

Positive controls:

no

Negative controls:

no

Preliminary study:

The test substance is stable at pH 7 and 9 (less than 10 % decomposition after 5 days at 50 °C).
The hydrolysis test could not be performed at pH 4 because the solubility of the test substance in the buffer solution at pH 4 is too low.

Transformation products:

no

% Recovery:

98.9

pH:

7

Temp.:

50 °C

Duration:

5 d

% Recovery:

99.2

pH:

7

Temp.:

50 °C

Duration:

2.4 h

% Recovery:

99.5

pH:

9

Temp.:

50 °C

Duration:

5 d

% Recovery:

99

pH:

9

Temp.:

50 °C

Duration:

2.4 h

Remarks on result:

hydrolytically stable based on preliminary test

Details on results:

The test substance is stable at pH 7 and pH 9 (less than 10 % decomposition after 5 days at 50°C.

Description of key information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments for hydrolytical stability were conducted for the triazine and the amine part, respectively.   Neither the triazine part nor the amine part of the compound is expected to significantly hydrolyze under envionmental conditions.

Additional information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments for hydrolytical stability were conducted for the triazine and the amine part, respectively.

CAS 80584 -91 -4

The hydrolysis behaviour of the triazine part was assessed in a "hydrolysis as a function of pH" study in accordance to EU-method C.7. The test item was exposed to three different buffer solutions at pH 4, 7 and 9. The solubility of test item depends on pH value. As the solubility at pH 4 is close to the detection limit, the test could not be carried out at this value. At pH 7 and pH 9 decomposition is less than 10 % after 5 days at 50 °C. Therefore the triazine part was considered to be hydrolytically stable.

CAS 102 -71 -6

The amine part of the compound is readily biodegradable and does not contain any functional groups that are susceptible to hydrolysis.

In conclusion, neither the triazine part nor the amine part of the compound is expected to significantly hydrolyze under envionmental conditions.