reaction mass of: tetrasodium 4-amino-6-(5-(2,6-difluoropyrimidin-4-ylamino)-2-sulfonatophenylazo)-5-hydroxy-3-(4-(sulfatoethylsulfonyl)phenylazo)naphthalene-2,7-disulfonate;tetrasodium 4-amino-6-(5-(4,6-difluoropyrimidin-2-ylamino)-2-sulfonatophenylazo)-5-hydroxy-3-(4-(2-sulfatoethylsulfonyl)phenylazo)naphthalene-2,7-disulfonate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998-04-21 to 1998-08-13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

GLP compliance:

yes

Type of method:

flask method

Water solubility:

ca. 624 g/L

Conc. based on:

test mat.

Temp.:

20 °C

pH:

5.9

Key result

Water solubility:

ca. 437 g/L

Conc. based on:

act. ingr.

Temp.:

20 °C

pH:

5.9

Remarks on result:

other: corrected for the purity of 70 % (w/w)

Table: Water solubility of the test substance

Experiment	2	3
Amount of test substance (g)	16.8748	16.8831
Stirring time at 20 °C	3	3
pH at room temperature	5.9	5.9
Water solubility	621.84	625.92

 

Average (experiments 2 to 3): 623.88 g/L

Standard deviation: 2.89 g/L

Relative standard deviation: 0.46 %

Purity-corrected value (70 % w/w): 437 g/L

 

Table: HPLC peak areas from the solubility experiment (dilution factor: 5000) and used standards.

Stirring time at 20 °C (h)	Peak areas (mV*min)	Concentrations (mg/l)	Peak areas (mV*min)
Experiment 2	Experiment 2	Standard B	Standard B
	276.659	234.84	261.289
3	275.638		260.153
Mean:	276.149		260.721
Experiment 2	Experiment 2	Standard B	Standard B
	283.246	262.52	296.109
3	280.152		294.632
Mean:	281.699		295.371

 

Conclusions:

The test substance Reactive Navy FC63805 is very soluble in water with a solubility of 624 g/L (purity-adjusted: 437 g/L) at 20°C and a pH of 5.9.

Executive summary:

The water solubility of the test substance was measured by HPLC in accordance with EU method A.6 and in compliance with GLP. The concentration of the substance was determined by comparison of HPLC peak area with an external standard.

The water solubility was determined to 624 g/l and corrected for the purity of 70 %, resulting in definitive solubility of 437 g/l (RSD: 0.5 %).

Description of key information

A study was performed to determine the water solubility of the test substance Reactive Navy FC63805 following EU method A.6 and in compliance with GLP, using the flask method and HPLC. The test substance is classified as very soluble in water with a solubility of 624 g/L (437 g/L corrected for 70 % purity w/w) at 20°C at a pH of 5.9.

Key value for chemical safety assessment

Water solubility:

437 g/L

at the temperature of:

20 °C

Additional information

The water solubility of the test substance Reactive Navy FC63805 was measured by flask method in combination with HPLC and the concentration of the substance was determined by comparison of HPLC peak areas with an external standard.

The water solubility was determined to 624 g/l and corrected for the purity of 70 %, resulting in a definitive solubility of 437 g/l (RSD: 0.5%) at 20°C and a pH of 5.9. The test substance is classified as very soluble in water at the test conditions.