2-Naphthalenesulfonic acid, 7-amino-4-hydroxy-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-, potassium sodium salt (1:?:?), coupled with diazotized 2-[(4-amino-5-methoxy-2-methylphenyl)sulfonyl]ethyl hydrogen sulfate

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experiment start date - 09 July 2003; Experiment end date - 12 July 2003; Study completion date - 28 August 2003.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.8 (Partition Coefficient)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

other: Calculation method

Partition coefficient type:

octanol-water

Specific details on test material used for the study:

Identity: FAT 40812/A
Batch: WP 8/03
Appearance: Solid, dark red-brownish powder
Expiration date: 23 April 2010
Storage: At room temperature at about 20 °C

Analytical method:

high-performance liquid chromatography

Type:

log Pow

Partition coefficient:

< -5.5

Remarks on result:

other: pH not available

Details on results:

Neither the HPLC-method according to OECD Guideline No. 117 nor the flask-shaking method according to OECD Guideline No. 107 were applicable for the determination of the partition coefficient of FAT 40'812. Thus, the log Pow-value for the test item was estimated from its solubility in n-octanol and in water, respectively. In the preliminary test, the n-octanol solubility of FAT 40'812/A was determined to be <1.2012 µg/mL. The water solubility of FAT 40812/A was found to be >419 g/L at room temperature using the flask shaking test.

Conclusions:

The calculated partition coefficient of the test substance between n-octanol and aqueous phase is: log Pow ≤-5.5

Executive summary:

In a GLP-compliant study, performed according to OECD guideline 117 and 107, the partition coefficient of the test substance between n-octanol and aqueous phase was determined. Neither the HPLC-method nor the flask-shaking method were applicable for the determination of the partition coefficient of the test substance. Thus, the log Pow-value for the test item was estimated from its solubility in n-octanol and in water. The calculated partition coefficient of the test substance between n-octanol and aqueous phase is log Pow ≤-5.5.

Description of key information

In a GLP-compliant study, performed according to OECD guideline 117 and 107, the partition coefficient of the test substance between n-octanol and aqueous phase was determined. Neither the HPLC-method nor the flask-shaking method were applicable for the determination of the partition coefficient of the test substance. Thus, the log Pow-value for the test item was estimated from its solubility in n-octanol and in water. The calculated partition coefficient of the test substance between n-octanol and aqueous phase is log Pow = <-5.5.

Key value for chemical safety assessment

Log Kow (Log Pow):

-5.5

at the temperature of:

20 °C