2,6-difluoro-3-[(propylsulfonyl)amino]benzoic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Feb 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study conducted under GLP conditions and in acc. with OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,6-difluoro-3-(propane-1-sulfonamido)benzoic acid

EC Number:

700-740-0

Cas Number:

1103234-56-5

Molecular formula:

C10H11F2NO4S

IUPAC Name:

2,6-difluoro-3-(propane-1-sulfonamido)benzoic acid

Details on test material:

-Substance type: organic
-Physical state: solid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

Prior to the definitive test a non-GLP range finding test with the nominal
concentrations of 1, 10 and 100 mg/1 was performed.
Since no significant effects were observed in this range finding test only a limit test
with the nominal concentration of 100 mg/1 was performed as a definitive test.
Due to the limited water solubility of the test substance, only the water
accommodated fraction was tested.
This water accommodated fraction was prepared by addition of the respective
amount of an acetonic stock solution to an empty glass vessel. After complete
evaporation of the solvent, the aerated Oaphnia medium (Tables 1 and 2) was
added. The test solution was moderately stirred overnight followed by filtration
(MILLIPORE AP15 glass fibre filter). The resulting water accommodated fraction
was used in the test.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 79.8 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

79.8 mg/L

Applicant's summary and conclusion

Executive summary:

The median effective concentration (EC50) of SAC-Sulfonamidsaure to Daphnia magna was investigated under static exposure conditions over a period of 48 h. The only applied nominal test concentration was 100 mg/1. 40 individual Daphnia divided into 2 test vessels were exposed to the test substance. Chemical analyses of the test substance concentrations were conducted at the beginning of the test as well as after 24 and 48 h of incubation, respectively. The analyses revealed that the test material was only partly dissolved and that the test substance concentrations were satisfactorily maintained over the whole exposure period. Using the water accommodated fraction of SAC-Sulfonamidsaure no toxic effects (=<10% immobilization) were observed after 24 and 48 h of exposure. No toxic effects were also observed in the control after 24 and 48 h of exposure. Based on these results it can be concluded that the 24 and 48 h EC₅₀ values of SAC-Sulfonamidsaure were >100 mg/1 with respect to the loading rate. Based on the analytically determined concentrations the 24 and 48 h EC50 values were >79.9 and >79.8 mg/1, respectively. The no-observed-effect concentration (NOEC) for 24 and 48 h of incubation was 100 mg/1 with respect to the loading rate. Based on the analytically determined concentrations the 24 and 48 h NOEC were 79.9 and 79.8 mg/1, respectively. 100% immobilization was not detected