(R)-1-hydroxy-1-phenylacetone

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to a BASF internal test method, which in principle was similar to the Inhalation Hazard Test as described in Annex V of the OECD TG 403 81981). GLP was not compulsory at the time the study was conducted.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

(1981; Annex V: Inhalation Hazard Test)

Principles of method if other than guideline:

similar to H.F. Smyth et al. (1962) Am. Ind. Hyg. Ass. J. 23 (95-107)

GLP compliance:

no

Remarks:

GLP was not compulsory at the time the study was conducted

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(R)-1-hydroxy-1-phenylacetone

EC Number:

217-285-4

EC Name:

(R)-1-hydroxy-1-phenylacetone

Cas Number:

1798-60-3

Molecular formula:

C9H10O2

IUPAC Name:

(1R)-1-hydroxy-1-phenylpropan-2-one

Details on test material:

- Name of test material: (-)-Phenylacetylcarbinol
- Physical state: liquid
- Analytical purity: 67.4 %

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Fa. Wiga Versuchstieranstalt, Sulzfeld, Germany
- Age at study initiation: 7 to 10 weeks
- Weight at study initiation: male 180 - 250 g, female 180 - 250 g
- Housing: 3 animals /cage, Becker D III cages
- Diet (e.g. ad libitum): Pellets, Herilan MRH (Eggersmann KG, Rinteln), ad libitum
- Water (e.g. ad libitum): ca. 250 mL/cage
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2°C
- Humidity (%): 55 ± 5 %
- Photoperiod (hrs dark / hrs light): 12 h/12 h

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

The product was filled in a glass bottle to a height of 5 cm (fritted glass flask; pore size 90- 150 µm and diameter 30 mm) and the weight was determined.
A stream of 200 L/h compressed air was supplied to the fritted glass flask containing the product, which had been placed in a water bath maintained at 20±1°C by a thermostat.
The mixture of air and test substance generated in this way was passed through a glass distributor to 6 glass tubes in which 3 male and 3 female animals had been placed. The emerging mixtures of vapor and air were exhausted. After 30 minutes, the fritted glass flask was replaced by a new one filled with fresh test substance as described above. This receiver was then used for the whole remaining time of the test.

Analytical verification of test atmosphere concentrations:

no

Remarks:

(test item was determined gravimetrically, therefore at the end of the exposure period the glass flask was reweighted)

Duration of exposure:

7 h

Concentrations:

maximum concentration of 2 tests: 0.25 mg/L (related to 7 hrs exposure time)

No. of animals per sex per dose:

6 per test (in 2 independent tests)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations for symptoms and mortality: daily
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 0.25 mg/L air

Based on:

test mat.

Exp. duration:

7 h

Remarks on result:

other: no mortality was seen

Mortality:

no mortality observed

Clinical signs:

other: wiping of the mouth

Body weight:

no data

Gross pathology:

no findings