(R)-1-hydroxy-1-phenylacetone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(R)-1-hydroxy-1-phenylacetone

EC Number:

217-285-4

EC Name:

(R)-1-hydroxy-1-phenylacetone

Cas Number:

1798-60-3

Molecular formula:

C9H10O2

IUPAC Name:

(1R)-1-hydroxy-1-phenylpropan-2-one

Details on test material:

- Name of test material (as cited in study report): (-)-Phenylacetylcarbinol, roh
- Analytical purity: 65.47 %
- Lot/batch No.: Pa.222/26

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration, 100 mg/l (nominal) was prepared by stirring the test substance in the M4 medium for a period of 10 minutes at 20 +/- 2 °C. Serial dilutions of this highest test concentration were subsequently prepared in order to obtain the desired test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2- 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 - 3.20 mmol/l

Test temperature:

20 - 21 °C

pH:

7.5 - 8.2

Dissolved oxygen:

8.3 - 9.1 mg/l

Nominal and measured concentrations:

Nominal test concentrations: 12.5, 25, 50 and 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to ISO 10706
- Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/l
- Ca/mg ratio: approx. 4 : 1
- Conductivity: 550 - 650 microS/cm

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: artificial light, type warm white (e .g. OSRAM L58 W31), 1 - 8 microE/(m2*s) at a wave length of 400 - 700

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Due to the results of the test no statistical evaluation of the data was performed.

Any other information on results incl. tables

No immobilization of Daphnia was observed in any of the tested  concentrations of (-)-Phenylacetylcarbinol, roh -

Effective concentrations of the test substance after 24 h and 48 h  (nominal values).
Time /  EC0    /  EC50   /  EC100   /
[h]  / [mg/l]  / [mg/l]  /  [mg/l]  /
24   /  >100   /  >100   /   >100   /
48   /  >100   /  >100   /   >100   /

All results refer to nominal test concentrations of  (-)-Phenylacetylcarbinol, roh -