Registration Dossier
Registration Dossier
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EC number: 219-909-0 | CAS number: 2568-90-3
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- no
Test material
- Reference substance name:
- 1,1'-[methylenebis(oxy)]dibutane
- EC Number:
- 219-909-0
- EC Name:
- 1,1'-[methylenebis(oxy)]dibutane
- Cas Number:
- 2568-90-3
- Molecular formula:
- C9H20O2
- IUPAC Name:
- 1,1'-[methylenebis(oxy)]dibutane
- Details on test material:
- - Batch number: Charge 825
- Date of production: 22 Sep 1999
- Molecular formular: no details
- Molecular weight [g/mol]: no details
- Aggregate state: liquid
- Colour: colourless
- Purity of the test substance: 99.78 area-%
- Impurities: no details
- Homogeneity: yes
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage.
- Preparation of inoculum for exposure: inoculum was washed and aerated for 24 hours before dosing into the test vessels
- Pretreatment: none
- Concentration of sludge: 30 mg/l dry substance - Duration of test (contact time):
- 34 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: room temperature
- pH: 7.2-7.5
SAMPLING
- Sampling frequency: continuously for 28 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
- Other: inhibition of the inoculum
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 30 - 40
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 40 - 50
- Sampling time:
- 34 d
- Details on results:
- - Duration of adaption phase: 13 days
- Duration of degradation phase: 12 days
- Degradation of reference substance after 14 days (BOD/ThOD): 70-80%
Applicant's summary and conclusion
- Interpretation of results:
- other: partly or moderately biodegradable and not readily biodegradable according to OECD criteria
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