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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 2013 - July 2013
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
yes
Remarks:
The abiotic sterile control was prepared with deionised water and not with medium. This procedure, according to standard operating procedures, is chosen to reduce the possibility of any microbial activity. It was wrongly indicated in the study plan.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Tert-butyl 3-oxoazetidine-1-carboxylate
EC Number:
627-725-0
Cas Number:
398489-26-4
Molecular formula:
C8H13NO3
IUPAC Name:
Tert-butyl 3-oxoazetidine-1-carboxylate
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
Identification PAM-Azetidinone

Chemical name 3-Oxoazetidine-1-carboxylic acid tert-butyl ester

Batch no RC10050008

CAS no. 398489-26-4

Molecular formula C8H13NO3

BMG sample no. M1302-00844-01

Physical form Solid

Purity 99.4% (w/w)

Theoretical oxygen demand 2.056 mg O2/mg (calculated according to chapter 5.2 assuming nitrification)

Test substance storage At room temperature, protected from light

Stability Stable under storage conditions

Expiry date 22 June 2013

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Duration of test (contact time):
ca. 28
Initial test substance concentration
Initial conc.:
ca. 100 mg/L
Based on:
ThOD/L

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
ca. 15
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
PAM-Azetidinone is not biodegradable in the Manometric Respirometry Test.
Executive summary:

The biodegradability of PAM-Azetidinone exposed to microorganisms derived from activated sludge of a municipal sewage treatment plant was investigated under aerobic static exposure conditions.

No significant biodegradability (15%) of PAM-Azetidinone based on O2 consumption was observed during 28 days as compared to the theoretical oxygen demand (ThOD).

The procedure control sodium benzoate reached 80% biodegradation after 14 days, thus confirming suitability of inoculum and test conditions.

The calculated biodegradation based on DOC measurements reached 19% for PAM-Azetidinone and 99% for sodium benzoate, respectively. The data confirm that no significant biodegradability occurred.

PAM-Azetidinone is not biodegradable in the Manometric Respirometry Test.