COASOL 290 PLUS

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

28 d

Initial conc.:

2 mg/L

Based on:

test mat.

Initial conc.:

4 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (O2 consumption)

Value:

34

Sampling time:

5 d

Parameter:

% degradation (O2 consumption)

Value:

57

Sampling time:

15 d

Parameter:

% degradation (O2 consumption)

Value:

80

Sampling time:

28 d

Results with reference substance:

The reference substance biodegradation was as follows (based on oxygen consumptionl)
5 days: 46%
15 days:65%
28 days: 85%

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

This substance was readily biodegradable under the conditions of the assay

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Study period:

2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: OECD 306, Biodegradability in Sea Water

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

other: Sea Water; obtained from Huttoft on the East Coast of the UK, 19-08-2009

Duration of test (contact time):

ca. 28 d

Initial conc.:

ca. 1 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Parameter:

% degradation (O2 consumption)

Value:

ca. 68

Sampling time:

28 d

Oxygen depletion in the control vessels was 12% of the initial dissolved oxygen concentration and the residual concentration remained at 2.80 mg O2/L or greater in all vessels, thereby satisfying the validation criteria forthe oxygen depletion. Due to low aqueous solubility of the test material, following the recommendations of the International Standards Organisation (ISO 1995), the test material was adsorbed onto an inert support prior to addition to the test vessels.

The test material attained 68% degradation after 28 days and. therefore, can be considered to have the potential for biodegradation after 28 days in the marine environmnent. There was a lag phase prior to the start of degradation of about 1 day.

The toxicity control attained 63% degradation after 14 days and 77% after 28 days. The test material was not toxic to the marine organisms.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test material attained 68% degradation after 28 days and therefore can be considered to have the potential for biodegradation in the marine environment under the terms and conditions of OECD Guideline No. 306

Description of key information

COASOL 290 PLUS is a reaction mass of dibutyl glutarate and dibutyl adipate, while COASOL is a reaction mass of the very similar isomers diisobutyl glutarate, diisobutyl succinate, and diisobutyl adipate. The results from a supporting study with COASOL are applied to COASOL 290 PLUS (read-across). The available biodegradation studies demonstrate that COASOL 290 PLUS was readily biodegradable in an OECD 301D Closed Bottle Test, whereas COASOL showed extensive biodegradation in an OECD 306 Biodegradability in Seawater Test.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

COASOL 290 PLUS is a reaction mass of dibutyl glutarate and dibutyl adipate, while COASOL is a reaction mass of the very similar isomers diisobutyl glutarate, diisobutyl succinate, and diisobutyl adipate. Thus, results from studies for COASOL are applied to COASOL 290 PLUS (read-across). COASOL 290 PLUS was readily biodegradable in the OECD 301D test, reaching 80% in 28 days. The test substance also showed extensive biodegradation the the OECD 306 Biodegradability in Seawater test, reaching 68% in 28 days.