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EC number: 278-860-3 | CAS number: 78182-00-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted in compliance with GLP regulations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (2-hydroxyethyl)(3-hydroxypropyl)dimethylammonium chloride
- EC Number:
- 278-860-3
- EC Name:
- (2-hydroxyethyl)(3-hydroxypropyl)dimethylammonium chloride
- Cas Number:
- 78182-00-0
- Molecular formula:
- C7H18NO2.Cl
- IUPAC Name:
- 3-hydroxy-N-(2-hydroxyethyl)-N,N-dimethylpropan-1-aminium chloride
- Details on test material:
- - Name of substance (as cited in study report): DMAC TROCKEN FLUESSIG
- Chemical name: 1-Propanaminium, 3-hydroxy-N-(2-hydroxyethyl)-N, N-dimethyl-, chloride
- Molecular formular: C7 H18 N O2 Cl
- Molecular weight (g/mol): 183.7
- Aggregated state: solid
- Density (g/ml): 1.12
- Water solubility: miscible
- Colour: light brown
- Purity of test substance: 98.9 %
- Homogeneity: yes
- Instability against: temperature > 100 °C
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Inoculum: Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage.
- Concentration of dry substance 30 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 70 - 80
- Sampling time:
- 28 d
- Details on results:
- - Duration of adaption phase (d): 10d
- Duration of degradation phase (d): 7
- Degradation of test substance at end of 10-d-window (% BOD/ThOD): 80 - 90 (Nitrification not considered)
- Degradation degree of test substance after 28 d (% BOD/ThOD): 70 - 80
- Degradation degree of test substance in abiotic control at end of test (% BOD/ThOD): 0 - 10
- Kinetic of test substance mean (in %):
= 0 after day 1
= 6 after day 11
= 18 after day 12
= 52 after day 13
= 60 after day 14
= 64 after day 15
= 86 after day 28
BOD5 / COD results
- Results with reference substance:
- - Degradation degree of reference substance after 14 days (% BOD/ThOD): 80 - 90
- Degradation degree in inhibition control after 14 days (% BOD/ThOD): 70 - 80
Applicant's summary and conclusion
- Interpretation of results:
- readily biodegradable
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