1,4,7-trioxacyclotridecane-8,13-dione

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-11-17 to 1999-12-08

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,3,7,8-tetrahydro-2,5,8-benzotrioxacycloundecine-1,9-dione

EC Number:

229-554-3

EC Name:

2,3,7,8-tetrahydro-2,5,8-benzotrioxacycloundecine-1,9-dione

Cas Number:

6607-34-7

Molecular formula:

C10H16O5

IUPAC Name:

1,4,7-Trioxacyclotridecane-8,13-dione

Details on test material:

- Name of test material (as cited in study report): Adipic acid, Cyclic ester with diethylenglycol
- Analytical purity: > 90%
- Impurities (identity and concentrations): Diethylenglycol <10% ; water<2%
- Purity test date: Report of the sponsor of Jan 1999
- Lot/batch No.: B47

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Laboratory wastewater plants treating municipal and synthetic sewage
- Preparation of inoculum for exposure: Inoculum was pre-aerated two days before the start of the test
- Initial cell/biomass concentration: 30 mg/L dry substance

Duration of test (contact time):

21 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

37 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Test temperature: room temperature

TEST SYSTEM
- Culturing apparatus: batch system, 1 L total test volume
- Number of culture flasks/concentration: 2
- Test performed in open system: yes

SAMPLING
- Sampling frequency: every 1-3 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
- Other: in addition an inhibition control and adsorption control was tested in parallel

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

1

Sampling time:

1 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

17

Sampling time:

3 d

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

61

Sampling time:

5 d

% Degradation 4

Parameter:

% degradation (DOC removal)

Value:

87

Sampling time:

10 d

% Degradation 5

Parameter:

% degradation (DOC removal)

Value:

100

Sampling time:

14 d

BOD5 / COD results

Results with reference substance:

DOC-decrease of reference control:
1 day: 3%
3 days: 22%
5 days: 97%

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

1) Degradation degree of the test substance >70% within 28 days: yes; 2) 10-day window met: yes; 3) No pre-adapted inoculum used: yes

Interpretation of results:

readily biodegradable