1,2-Benzenedicarbonitrile, 3-nitro-

Particle size distribution (Granulometry)

Administrative data

Endpoint:

particle size distribution (granulometry)

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study experimental phase 2013-10-28 to 2014-01-06. Study report complete 2014-05-23.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Meets the criteria for classification as reliable without restriction according to Klimisch et al (1997).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 110 (Particle Size Distribution / Fibre Length and Diameter Distributions)

Deviations:

no

Principles of method if other than guideline:

The Test Item was examined by Optical (Light) Microscopy and Laser Diffraction after the test sampe had been sieved through a 300um sieve.
Optical (Light) Microscopy was used to characterise the Test Item to allow a better understanding of its physical characteristics prior to laser diffraction analysis. This is a visual assessment to provide additional supporting information, e.g. the presence of agglomeration etc, to allow interpretation of the laser diffraction data and understand the limitations of the laser diffraction data.
Laser Diffraction was used to determine the particle size distribution of the Test Item and assess the MMAD (mass median aerodynamic diameter).

GLP compliance:

yes

Type of distribution:

mass based distribution

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

450-650-8

EC Name:

-

Cas Number:

51762-67-5

Molecular formula:

C8H3N3O2

IUPAC Name:

3-nitrobenzene-1,2-dicarbonitrile

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Results and discussion

Mass median aerodynamic diameter:

243.142 µm

Particle size

Particle size 1

Percentile:

D50

Mean:

289.737 µm

Remarks on result:

other: Migrated from fields under 'Mass median diameter' as D50 percentile. No source field for Standard deviation.

Particle size 2

Percentile:

D10

Mean:

< 97.846 µm

St. dev.:

0.667

Particle size 3

Percentile:

D50

Mean:

< 289.737

St. dev.:

1.005

Particle size 4

Percentile:

D90

Mean:

< 617.105

St. dev.:

2.066

Any other information on results incl. tables

Optical (Light) Microscopy

By direct observation the Test Item was observed to be a pale yellow powder.

With the 5x objective the particles appeared to be mostly irregularly shaped (no lines of symmetry) with a wide variety of sizes visible. With the 10x objective the particles appeared to be mostly irregularly shaped (no lines of symmetry) with a wide variety of sizes visible.

Coefficient of variation for d50 and d10 are less than 3%; d90 is less than 5%. Thus repeatability of the characteristic particles in the size distribution are within acceptable limits as per the ISO 13320-1 test standard.

Applicant's summary and conclusion

Conclusions:

By direct observation the Test Item was observed to be a pale yellow powder. With the 5x objective (light microscopy) the particles appeared to be mostly irregularly shaped (no lines of symmetry) with a wide variety of sizes visible. With the 10x objective the particles appeared to be mostly irregularly shaped (no lines of symmetry) with a wide variety of sizes visible.

The MMAD of the test material is 243.142um.
10% of the material has a diameter of <97.846um
50% of the material has a diameter of <289.737um
90% of the material has a diameter of <617.105um

Inhalable particles, which can be inhaled by nose and mouth, have a particle size of <100um. Therefore at least 10% of the test material has a particle size which can be inhaled by nose and mouth.

Executive summary:

Introduction

This study was undertaken to determine the particle size distribution of the test material. The Test material was examined by Optical (Light) Microscopy and Laser Diffraction after the test sampe had been sieved through a 300um sieve. Optical (Light) Microscopy was used to characterise the Test Material to allow a better understanding of its physical characteristics prior to laser diffraction analysis. This is a visual assessment to provide additional supporting information, e.g. the presence of agglomeration etc, to allow interpretation of the laser diffraction data and understand the limitations of the laser diffraction data. Laser Diffraction was used to determine the particle size distribution of the Test Material and assess the MMAD (mass median aerodynamic diameter).

Results & Conclusions

By direct observation the Test Item was observed to be a pale yellow powder. With the 5x objective (light microscopy) the particles appeared to be mostly irregularly shaped (no lines of symmetry) with a wide variety of sizes visible. With the 10x objective the particles appeared to be mostly irregularly shaped (no lines of symmetry) with a wide variety of sizes visible.

The MMAD of the test material is 243.142um. 10% of the material has a diameter of <97.846um. 50% of the material has a diameter of 289.737um. 90% of the material has a diameter of 617.105um.

Inhalable particles, which can be inhaled by nose and mouth, have a particle size of <100um. Therefore at least 10% of the test material has a particle size which can be inhaled by nose and mouth.