N,N,N-triethylethanaminium 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulfonate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: ICCVAM Test Method Evaluation Report: Appendix G: ICCVAM Recommended Protocol for Future Studies Using the Hen's Egg Test-Chorionallantoic Membrane (HET-CAM) Test method, November 2006

Principles of method if other than guideline:

The hen's egg test was modified as an alternative to the rabbit's eye irritation/corrosion test. The extra-embryonal blood vessel system of the incubated chicken egg is used to observe the acute toxicological effects of irritant/corrosive test substances. This test was performed by using the chorionallantoic membrane (HET-CAM, 8d incubation) of chicken eggs

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

25628-80-4

IUPAC Name:

25628-80-4

Constituent 2

Reference substance name:

tetraethylazanium nonafluorobutane-1-sulfonate

EC Number:

700-536-1

Cas Number:

25628-08-4

Molecular formula:

C12H20F9NO3S

IUPAC Name:

tetraethylazanium nonafluorobutane-1-sulfonate

Constituent 3

Reference substance name:

N,N,N-triethylethanaminium 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulfonate

IUPAC Name:

N,N,N-triethylethanaminium 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulfonate

Details on test material:

content: 97.7%

Test animals / tissue source

Species:

other: not applicable (in vitro study)

Strain:

other: not applicable (in vitro study)

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: not applicable (in vitro study)

Duration of treatment / exposure:

single treatment

Observation period (in vivo):

Effects are measured by the onset of haemorrhage, vessel lysis or coagulation during the first 300 seconds after application

Number of animals or in vitro replicates:

not applicable - in vitro test (4 eggs were used)

Results and discussion

In vivo

Results

Irritation parameter:

other: Irritation score (IS)

Basis:

mean

Time point:

other: 300 sec

Score:

4

Max. score:

37

Reversibility:

other: not applicable - in-vitro test

Other effects:

no data

Any other information on results incl. tables

                                     HET-CAM              Index

Negative Control       NaCl 0.9%              0 Non irritant

Positive Control        SDS 1%                 11 Strong irritant

Perfluorbutansulfonsäure-

Tetraethylammonium-Salz                          4 Slight irritant

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information

Executive summary:

Hen' s egg tests are used to determine the potential irritantlcorrosive property of the test compound using an in vitra alternative to the Draize methodology (OECD405).

Tetraethylammonium perfluorobutanesulfonate was tested by observing possible adverse changes after application onto the chorionallantoic membrane (HET-CAM).

After the application of the test item blood vessels and albumen were examined and scored for the following irritant effects: vasodilation, slight haemorrhage; vessel lysis, strong haemorrhage; blood coagulation, albumen coagulation for a period of 300

seconds. 300 µg of the test item was applied per egg directly on the chorionallantoic membrane.

Tetraethylammonium perfluorobutanesulfonate was identified as "slight irritant" to the chorionallantoic membrane (Irritation Score (IS) = 4) under the conditions of this assay.