Cyclopropanecarbonitrile, 1-amino-, hydrochloride (1:1)

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Acceptable study report well documented that meets basic scientific principles, comparable to guideline OECD 471 (Bacterial Reverse Mutation Assay).

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1-Amino-1-cyclopropanecarbonitrile hydrochloride

EC Number:

629-718-8

Cas Number:

127946-77-4

Molecular formula:

C4H7N2Cl

IUPAC Name:

1-Amino-1-cyclopropanecarbonitrile hydrochloride

Constituent 2

Reference substance name:

1-Cyanocyclopropanaminium

IUPAC Name:

1-Cyanocyclopropanaminium

Constituent 3

Reference substance name:

1-aminocyclopropane-1-carbonitrile hydrochloride

IUPAC Name:

1-aminocyclopropane-1-carbonitrile hydrochloride

Method

Target gene:

Target gene S.Typhimurium
Target gen E. Coli

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 100

Additional strain / cell type characteristics:

not specified

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 1535

Additional strain / cell type characteristics:

not specified

Species / strain 3

Species / strain / cell type:

S. typhimurium TA 97

Additional strain / cell type characteristics:

not specified

Species / strain 4

Species / strain / cell type:

S. typhimurium TA 98

Species / strain 5

Species / strain / cell type:

E. coli WP2 uvr A pKM 101

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

Liver microsomal enzymes from rats pretreated with xenobiotics.

Test concentrations with justification for top dose:

The concentration 0, 100, 300, 1000, 3000 and 6000µg/plate were chosen for the main experiments.

Vehicle / solvent:

Vehicle used: distilled water.

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-Aminoanthracene (2AA)

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium, other: TA 97, TA 98, TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: strain/cell type: TA 1535

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
positive

In view of theses data, ACC is detectably mutagenic in bacteria.