Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-076-7 | CAS number: 51-03-6
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Published study
Data source
Reference
- Reference Type:
- publication
- Title:
- Lack of genotoxicity of Piperonyl Butoxide
- Author:
- Butler, W.H., Gabriel, K.L., Preiss, F.J., Osimitz, T.G.
- Year:
- 1 996
- Bibliographic source:
- Mutation research 371, 249-258
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- .
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Piperonyl butoxide
- IUPAC Name:
- Piperonyl butoxide
- Reference substance name:
- 2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether
- EC Number:
- 200-076-7
- EC Name:
- 2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether
- Cas Number:
- 51-03-6
- Molecular formula:
- C19H30O5
- IUPAC Name:
- 2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether
- Details on test material:
- Blend FEP 100
Purity 90%
Constituent 1
Constituent 2
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 from Aroclor 1254-induced male rats
- Test concentrations with justification for top dose:
- 0. 100, 333, 667, 1000, 3333, 5000 µg/mL
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: All strains (with S9) 2-Aminoanthracene; Without S9: TA 98, TA1538: 4-nitrofluorene, TA100, TA1535: Sodium azide, TA1537 ICR-191
- Details on test system and experimental conditions:
- Preincubation method,
Preincubation time 20 min - Evaluation criteria:
- Positive test result defined as a positive dose response.
- Statistics:
- Not required
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Piperonyl butoxide was not genotoxic - Executive summary:
In a micriobial mutagenicity test with Salmonella Typhimurium TA98, TA100, TA1535, TA1537 and TA1538 the test substance Piperonyl butoxide was tested both in the presence and absence of rat-liver S9 .
No mutagenic activiyt and no cytotoxicty were noted at dose levels of up to 5000 µg/plate.
Piperonyl was not genotoxic.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
