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EC number: 221-242-5 | CAS number: 3039-83-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-03-05 to 1992-03-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 101.6 mg/L (nominal concentration)
- Sampling method: high performance liquid chromatography (HPLC)
- Sample storage conditions before analysis: Test concentrations were verified by chemical analysis. Water samples (~ 50 mL) were taken from the control and test level (replicates pooled) at 0 hours and 48 hours, membrane filtered (0.45 µm) and stored at ca. -20 °C prior to being sent for analysis. - Vehicle:
- no
- Details on test solutions:
- The test substance was dissolved in test water to give an stock solution of 400 mg/L (equivalent to 101.6 mg active ingredient/L).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Straus
- Source: Institute National de Recherche Chimique Appliquée (IRChA), France
- Age at study initiation: less than 24 hours - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 171 mg CaCO3/L
- Test temperature:
- 21 °C
- pH:
- 7.9 - 8.0
- Dissolved oxygen:
- 7.9 - 8.3 mg O2/L
- Nominal and measured concentrations:
- nominal: 400 mg/L (= 101.6 mg active ingredient/L)
measured: 94 mg active ingredient/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass jar
- Type: open
- fill volume: 200 mL
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Laboratory tap water filtered, dechlorinated and softened by passage through an Elga® water purification system
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light/8 hours dark
EFFECT PARAMETERS MEASURED: EC50; Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No
- Mortality of control: No
- Other adverse effects control: none
- Abnormal responses: No - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions of the present acute toxicity study in Daphnia magna with the 25.4 % aqueous test solution according to OECD 202, the 48 h EC50 (immobilisation) value was > 100 mg/L (based on active ingredient).
- Executive summary:
The potential of the test item to cause acute toxicity in daphnia magna was investigated in a study conducted in accordance with OECD 202 and EU method C2 under GLP conditions. For this purpose, young daphnids were exposed under static conditions for 48 hours to one concentration of the test item dissolved in water. The incidence of immobilisation was recorded for the test and control group at 24 hours and at 48 hours and the following values determined:
EC50 (24 h) > 100 mg/L
EC50 (48 h) > 100 mg/L
NOEC (24 h): ≥ 100 mg/L
NOEC (48 h): ≥ 100 mg/L
All validity criteria were met. All results were expressed in terms of nominal concentrations of active ingredient since the measured levels remained near nominal throughout the study (96 % of nominal at 0 hours and 89 % of nominal at 48 hours: average, 93 %).
Reference
Table 1: EC50 values
Time (h) |
EC50 (mg a.i./L) |
95% confidence limits (mg a.i./L) |
24 |
> 100 |
- |
48 |
> 100 |
- |
NOEC at 24 h: ≥ 100 mg a.i./L
NOEC at 48 h: ≥ 100 mg a.i./L
a.i. – active ingredient
Table 2: Cumulative immobilisation data for Daphnia magna exposed for 48 hours to test item
Nominal concentration (mg a.i./L) |
Measured concentration (mg a.i./L) |
Cumulative immobilised Daphnia magna (initial population: 10 per replicate) |
|||||||
24 hours |
48 hours |
||||||||
Total % |
Total % |
||||||||
Control |
R1 |
Control |
R1 |
0 |
0 |
0 |
0 |
0 |
0 |
R2 |
R2 |
0 |
0 |
0 |
0 |
0 |
0 |
||
100 |
R1 |
94 |
R1 |
0 |
0 |
0 |
0 |
0 |
0 |
R2 |
R2 |
0 |
0 |
0 |
0 |
0 |
0 |
||
R3 |
R3 |
0 |
0 |
0 |
0 |
0 |
0 |
||
R4 |
R4 |
0 |
0 |
0 |
0 |
0 |
0 |
R1 Replicate 1
R2 Replicate 2
R3 Replicate 3
R4 Replicate 4
Table 3: Measured concentrations (Mean values and percentages of nominal)
Nominal concentration (mg a.i./L) |
Number of samples analysed |
Measured concentration (mg a.i./L) |
% Nominal |
Control |
2 |
- |
- |
101.6 |
2 |
94.4 |
93 |
Description of key information
Under the test conditions of the present acute toxicity study in Daphnia magna with the 24.5 % aqueous test solution according to OECD 202, the 48 h EC50 (immobilisation) value was > 100 mg a.i./L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 100 mg/L
Additional information
The potential of the test item to cause acute toxicity in daphnia magna was investigated in a study conducted in accordance with OECD 202 and EU method C2 under GLP conditions. For this purpose, young daphnids were exposed under static conditions for 48 hours to one concentration of the test item dissolved in water. The incidence of immobilisation was recorded for the test and control group at 24 hours and at 48 hours and the following values determined:
EC50 (24 h) > 100 mg/L
EC50 (48 h) > 100 mg/L
NOEC (24 h): ≥ 100 mg/L
NOEC (48 h): ≥ 100 mg/L
All validity criteria were met. All results were expressed in terms of nominal concentrations since the measured levels remained near nominal throughout the study (96 % of nominal at 0 hours and 89 % of nominal at 48 hours: average, 93 %)
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