Heptan-1-ol

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline comparable to OECD 402

Data source

Reference

Reference Type:

publication

Title:

Contribution à l'étude toxicologique de l'alcool n-heptylique primaire,

Author:

R. Truhaut

Year:

1974

Bibliographic source:

Archive des maladies professionelles, de medecine du travail. T35, No 4-5, Avril-Mai (p 501-509)

Report date:

1974

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

Principles of method if other than guideline:

Draize et al., J pharmacol exp thera, 19477, 82, 377-390.

GLP compliance:

no

Test type:

other: Draize et al 1944

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Heptan-1-ol

EC Number:

203-897-9

EC Name:

Heptan-1-ol

Cas Number:

111-70-6

Molecular formula:

C7H16O

IUPAC Name:

heptan-1-ol

Details on test material:

Name: n-heptanol-1
Cas No. 111-70-6
Purity>97%

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

Rabbits were shaved on both the left and right side and also on the back, The skin exposure surface was therefore estimated between 200 cm2 to 350-400 cm2.

Administration / exposure

Type of coverage:

not specified

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

exposure surface= 250 to 350-400 cm2.

Duration of exposure:

2 weeks

Doses:

40 ml (1.3 g/kg) and 50 ml (2.7 g/kg)

No. of animals per sex per dose:

6 animals/dose

Control animals:

no

Details on study design:

50 ml of test item was administered on the skin surface. Animals were shaved on the back and the left and right sides. The dose-levels of 25 ml (20.5 g) , 40 ml ( 32.8g) and 50 ml (41.0g) were administrated by the cutaneous route. Half of the animals were sacrificed 2 weeks after the fisrt exposure and and the other half 6 weeks after. Major organs were sampled for histopathlogical examination.

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

25 ml (20.5 g equivalent 7.32 g/kg): no absorption, no mortality
40 ml(32.8g equivalent 11.7 g/kg): 1.3 g/kg interna dose: 2/6 dead animals
50 ml (41g equivalent 14.6 g/kg) : 2.7 g/kg internal dose: 4/6 dead animals.

Clinical signs:

other: At the absorbed dose-levels of 1.3 and 2.7 g/kg,tThe skin presented severe escars and sometimes suppurative lesions.

Gross pathology:

The results from histopathological examination revealed no alteration of the lung, the heart, liver, kidney, testes, pancreas and spleen. The brain presented spongiosis which was more severe in animals after 6 weeks exposure. The skin presented severe lesions of the derm with deep inflamatory infiltation.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Remarks:

Migrated information

Conclusions:

The LD50 of n-heptanol 1 is higher than 2000 mg/kg

Executive summary:

The acute dermal toxicity of n-heptanol was evaluated in rabbits according to a comparable draize method. The test item was applied to the skin of rabbits (6 animals/dose) at the dose-levels of 20.5, 32.8 and 41.0 g/ animal for 24 hours. Animals were then observed during 14 days for mortality, clinical signs, effects on body weight. Half of the rats were necropsied after 2 weeks and the other half after 6 weeks. 2/6 animals died at the dose-level of 32.8 g/animal (approx 11.7 g/kg) and 4/6 animals died at 2.7 g/animal (equivalent 14.6 g/kg). Lesions of the skin (caustic) and the brain were recorded at histopathological examination.

The LD 50 by dermal route was higher than 2 g/kg.