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EC number: 477-690-9 | CAS number: 874819-71-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005-01-12 - 2005-05-20
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Soil samples (100 g soil d.w.) are taken at intervals of 3 hours, 7, 14 and 28 days after application of the test item. Before the beginning of the test, the optimal glucose concentration was determined. Before the measurements, glucose (0.4 %) is added and mixed with the soil by means of a hand-stirrer. The prepared samples are placed in reaction flasks (500 mL).
The measurement of the oxygen consumption is carried out with a respirometer "BSB digi" (SELUTEC, Mossingen-Oschingen, Germany). The system records 360 02-consumption data in 24 hours. - Vehicle:
- yes
- Details on preparation and application of test substrate:
- The test item was dissolved in water and the test solution was then mixed with the soil in the mixing machine. Water was added to the soil to achieve a moisture of 45 % of WHC.
- Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20 °C ± 2 °C
- Moisture:
- Soil moisture: approximately 45 % of its maximum water holding capacity.
- Organic carbon content (% dry weight):
- 1.46
- Nitrogen content (% dry weight):
- 0.15
- Nominal and measured concentrations:
- 0.27 mg/kg d.w.
1.33 mg/kg d.w. - Reference substance (positive control):
- yes
- Remarks:
- Dinoterb
- Key result
- Duration:
- 28 d
- Dose descriptor:
- other: carbon transformation in soil
- Effect conc.:
- > 1 000 g/ha
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: carbon transformation
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1.33 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: carbon transformation
- Results with reference substance (positive control):
- The toxic standard dinoterb caused a reduction of the O2-consumption of > 25 % at the tested concentrations after 28 days and thus demonstrated the sensitivity of the test system.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of this study P 101/04 caused no short-term and long-term effects (OECD 217) on the carbon transformation in the field soil tested up to a concentration of 1.33 mg a.s. per kg soil d.w., equivalent to a field application rate of 1000 g a.s./ha.
- Executive summary:
The purpose of this study was to determine the effects of the test item on the activity of soil microflora with regard to carbon transformation in a laboratory test. The test was performed in accordance to the OECD guideline 217 (2000) by measuring the short-term substrate induced respiration. The method is based on the initial respiratory response of microbial populations to which a carbon and energy source has been added (substrate-induced respiration, SIR).
This assay is based on the determination of O2 consumption of soil samples after glucose-induced respiration in a closed system for at least 24 hours. Soil samples (100 g soil d.w.) are taken at intervals of 3 hours, 7, 14 and 28 days after application of the test item. Before the beginning of the test, the optimal glucose concentration was determined. Before the measurements, glucose (0.4 %) was added and mixed with the soil by means of a hand-stirrer. The prepared samples were placed in reaction flasks (500 mL). The measurement of the oxygen consumption was carried out with a respirometer "BSB digi" (SELUTEC, Mossingen-Oschingen, Germany).
No differences greater than 25 % to the control in O2 consumption were found for any test concentration of P 101/04 at any time interval in comparison to the respective control. The coefficients of variation during the experiment were within the demanded limit (control < 15%).
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005-01-12 - 2005-05-20
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The GLP study was conducted according to an internationally accepted guideline. All study parameters are based on the specific guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Determination of the nitrogen transformation (NO3-nitrogen production) in soil enriched with lucerne meal (concentration in soil 0.5 %). Comparison of test item treated soil with a non-treated soil. 3 replicates per treatment and concentration. NH4-nitrogen, NO3- and N02-nitrogen were determined by using the Autoanalyzer II (BRAN+LUEBBE). Sampling scheme: 0. 7, 14 and 28 days after treatment.
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- The test item was dissolved in water and the test solution was then mixed with the soil by means of a hand-stirrer. Water was added to the soil to achieve a moisture of 45 % of WHC. The incubation of the prepared soil was carried out in new plastic vessels (500 mL). The tops of the vessels used permitted an air exchange with negligible moisture leakage (< 1 % loss).
- Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20 ± 2 °C
- Moisture:
- approximately 45 % of WHC
- Nominal and measured concentrations:
- 0.27 mg/kg d.w.
1.33 mg/kg d.w. - Reference substance (positive control):
- yes
- Remarks:
- Dinoterb
- Key result
- Duration:
- 28 d
- Dose descriptor:
- other: soil nitrogen transformation
- Effect conc.:
- > 1 000 g/ha
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1.33 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Results with reference substance (positive control):
- In a separate study (study code: R 05 10 35 Nl) the reference item dinoterb caused effects greater than 25 % on the nitrogen transformation in a field soil at the tested concentrations 28 days after application.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of this study P 101/04 caused no short term and long-term effects (OECD 216) on the soil nitrogen transformation in the field soil tested up to a concentration of 1.33 mg a.s. per kg soil dry weight, equivalent to a field application rate of 1000 g a.s./ha.
- Executive summary:
The test was performed in accordance to the OECD guideline 216 (2000).
No differences greater than 25 % to the control in the nitrogen transformation were found for any concentration of P 101/04 in comparison to the respective control. The coefficients of variation during the experiment were within the demanded limit (control < 15%).
Based on the results of this study P 101/04 caused no short-term and long-term effects (OECD 216) on the soil nitrogen transformation in a field soil tested up to a concentration of 1.33 mg a.s. per kg soil dry weight, equivalent to a field application rate of 1000 g a.s. per ha.
Referenceopen allclose all
Days after application |
Control |
P101/04 0.27 mg/kg soild.w. |
P101/04 1.33 mg/kg soild.w. |
|||||
NO3-N [mg/kg soild.w.] |
CV[%] |
NO3-N [mg/kg soild.w.] |
CV[%] |
Deviation from control (%) |
NO3-N [mg/kg soild.w.] |
CV[%] |
Deviation from control (%) |
|
0 |
23.5 |
5.1 |
22.2 |
1.2 |
-5.7 |
21.9 |
6.3 |
-6.8 |
7 |
46.1 |
0.4 |
47.0 |
1.3 |
+2.0 |
47.0 |
3.6 |
+2.0 |
14 |
49.3 |
2.8 |
49.3 |
3.6 |
-0.1 |
48.4 |
1.6 |
-2.0 |
28 |
58.0 |
0.8 |
59.9 |
2.7 |
+3.2 |
57.9 |
2.1 |
-0.2 |
Description of key information
In two 28 d studies no effects were observed on soil micro organism at 1000 g/ha or 1.33 mg/kg soil dw.
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 1.33 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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