Reaction mass of sodium (methylbutyl and pentyl) sulfonate and sodium 1,2-bis(pentyloxycarbonyl)ethanesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: RWTH Aachen University, Institute for Environmental Research (Biology V)
Worringerweg 1
52074 Aachen
Germany

- Age at study initiation (mean and range, SD): < 24 h

- Method of breeding: M4 medium (according to OECD 202) until 48 hours before test start, thereafter, maintained in 100 % test medium (AAP test medium according to OECD 201) at the test temperature for at least 48 hours prior test start

- Source: held and bred in the test facility under standardised laboratory conditions, test animals derived from a healthy stock, not first brood progeny


- Feeding during test : no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

140 - 250 mg/L (CaCO3)

Test temperature:

20.3 – 20.5 °C (recorded continuously)

pH:

6.69 -7.52

Dissolved oxygen:

7.51 - 8.82 mg/L

Nominal and measured concentrations:

Control (0), 197.6, 296.4, 444.6, 666.9, 999.7 mg/L test item;
Control (0), 196.8, 295.1, 442.7, 664.0, 995.4 mg/L active substance

Recoveries for fresh samples were in the range from 106 to 109% of nominal, recoveries for spent samples were in the range from 111 to 115%. No test item was found in the control samples.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker (25 mL)
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 10 mL
- Volume of solution: 10 mL
- Aeration: no
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 4 + 3 additional vessels (1 for measuring of water parameters, 1 for analysis, 1 retain specimen)

- No. of vessels per control (replicates): 4 + 3 additional vessels (1 for measuring of water parameters, 1 for analysis, 1 retain specimen)

- Biomass loading rate: 2 mL test solution per Daphnia

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AAP medium according to OECD 201
- Culture medium different from test medium: same for last 48 hour prior to obtaining the neonates for this test
- Intervals of water quality measurement: 0 and 48 hours

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 light/dark phases
- Light intensity: on average 20 µE *m-2*s-1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
EC50 48 hours

VEHICLE CONTROL PERFORMED: no

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

602 mg/L

95% CI:

>= 534.6 - <= 679.3

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

other: immobilization

Details on results:

- Behavioural abnormalities: No abnormal behaviour or appearance was observed (e.g. trapping at surface of water or discoloration) in the control treatment and at the test concentrations ≤ 444.6 mg/L test item. At the test concentrations of 666.9 and 999.7 mg/L test item some daphnids trapped at surface of the test solution and showed active swimming after pressing the daphnids below the water surface.

- Mortality of control: none
- Other adverse effects control: none
- Immobilisation of control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no ne
- Effect concentrations exceeding solubility of substance in test medium: no

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Dose-response test: yes
- EC50: 1.76

Reported statistics and error estimates:

The EC10, EC20 and EC50 values were calculated by Probit analysis according to the maximum likelihood method. During the probit analysis the logarithm of the test concentrations was used to present the ECx results in the figure.

Statistical analysis was performed using the software ToxRat Professional (Version 3.3, Ratte, 20.10.2018)

Validity criteria fulfilled:

yes

Conclusions:

48h-EC50: 602 mg a.s./L

Executive summary:

The acute toxicity of Reaction mass of sodium (methylbutyl and pentyl) sulfonate and sodium 1,2-bis(pentyloxycarbonyl)ethanesulphonate to Daphnia magna was determined in a static GLP compliant test. The test was performed according to guideline OECD 202. The test was started with a control and nominal 197.6, 296.4, 444.6, 666.9, 999.7 mg/L test item corresponding to 0 (control), 196.8, 295.1, 442.7, 664.0, 995.4 mg/L active substance. For each test concentration and the control, 4 replicates with 5 daphinds each were set up in 10 mL vessels. Recoveries for fresh samples were in the range from 106 to 109% of nominal, recoveries for spent samples were in the range from 111 to 115%. Consequently, the test concentration was stable and the reported biological results are related to the nominal test item concentration.No test item was found in the control samples. The 48 h EC50 value is 602 mg active substance/L (95% C.I. 534.6 to 679.3 mg active substance /L).

The results of this study are considered to be reliable and relevant for the risk assessment of the substance registered with this dossier.

Description of key information

EC50 freshwater invertebrates: > 602 mg a.s. /L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

602 mg/L

Additional information

The acute toxicity of Reaction mass of sodium (methylbutyl and pentyl) sulfonate and sodium 1,2-bis(pentyloxycarbonyl)ethanesulphonate to Daphnia magna was determined in a static GLP compliant test. The test was performed according to guideline OECD 202. The test was started with a control and nominal 197.6, 296.4, 444.6, 666.9, 999.7 mg/L test item corresponding to 0 (control), 196.8, 295.1, 442.7, 664.0, 995.4 mg/L active substance. For each test concentration and the control, 4 replicates with 5 daphinds each were set up in 10 mL vessels. Recoveries for fresh samples were in the range from 106 to 109% of nominal, recoveries for spent samples were in the range from 111 to 115%. Consequently, the test concentration was stable and the reported biological results are related to the nominal test item concentration. No test item was found in the control samples. The 48 h EC50 value is 602 mg active substance/L (95% C.I. 534.6 to 679.3 mg active substance /L).

The results of this study are considered to be reliable and relevant for the risk assessment of the substance registered with this dossier.