1,4-bis(mesitylamino)anthraquinone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: study covers the required aspects but with weak documentation only

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,4-bis(mesitylamino)anthraquinone

EC Number:

204-155-7

EC Name:

1,4-bis(mesitylamino)anthraquinone

Cas Number:

116-75-6

Molecular formula:

C32H30N2O2

IUPAC Name:

1,4-bis(mesitylamino)anthraquinone

Study design

Inoculum or test system:

other: A mixed population of activated sewage sludge microorganisms.

Details on inoculum:

Source: The aeration stage of Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire.

Preparation:
The activated sludge was maintained at a temperature of 21 ± 1°C for 24 hours. An aliquot of the sludge was added to 10 L of dilution water to give a suspended solids level at 111.35 mg/L (100mg SS/L on dilution for the test) and aerated fo seven days prior to the start of the test to reduce the oxygen demand of the control.

Usage Rate: Equivalent to 100 mg dryweight/l.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

30 mg/L

Based on:

test mat.

Details on study design:

Criteria: Oxygen consumption measured by direct manometer reading.
Agitation: By magnetic stirrers
Temperature: 25 +-1 °C
Lighting: The test was carried out in darkness.
Observations: Manometer volumes were recorded daily.
Study Dates: 17th July - 14 August 1990

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

1 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

4

Sampling time:

8 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

9

Sampling time:

16 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

14

Sampling time:

24 d

% Degradation 5

Parameter:

% degradation (O2 consumption)

Value:

21

Sampling time:

21 d

BOD5 / COD results

Results with reference substance:

Aniline attained 48% biodegradation after 7 days and 71% after 14 days thereby confirming the suitability of the inoculum and culture conditions. Total Organic Carbon (TOC) analysis showed that aniline attained 95% degradation after 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The test item is not inherently biodegaradable

Executive summary:

A MITI test (according to Guideline OECD 302 C) was performed due to the low solubility of the test material in water. COD and TOC analysis were not carried out for this reason. 21% degradation after 28 days were found calculated from oxygen uptake and cannot therefore, be considered as inherently biodegradable under the strict terms and conditions of the OECD Guidelines.