7-acetamido-4-hydroxy-3-[[4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2-sulphonic acid, sodium salt

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1990-02-08 to 1991-02-22

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Provenienza:Hoechst wastewater biological treatment plant
- Concentration of sludge: 10 g/L, 1020 mg/L of dry weight

Duration of test (contact time):

ca. 28 d

Initial conc.:

ca. 44 other: mg C/L

Based on:

DOC

Initial conc.:

122 other: mgO2/L

Based on:

COD

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: according to OECD guideline.
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 22 °C
- pH: 7.3
- pH adjusted: yes with NaOH
- Aeration of dilution water: no

TEST SYSTEM
- Culturing apparatus: according to OECD guideline.

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no

Key result

Parameter:

% degradation (DOC removal)

Value:

ca. 4

Sampling time:

28 d

Details on results:

After 29 days of treatment the substance was degraded less than 10%. (~4%)

Results with reference substance:

No test with reference substance was performed.

Biodegradation within 28 days

time	elimination	biodegradation	mean
3h	0%	0%	< 10%
7 days	2%	2%
14 days	-8%	-8%
21 days	-2%	-2%
28 days	4%	4%

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

After 29 days of treatment the substance was degraded less than 10%. (~4%). The test substance is considered to be not inherently biodegradable.

Executive summary:

The test substance was tested by OECD 302B (Zahn-Wellens test). After 29 days of treatment the substance was degraded less than 10%. (~4%). The test substance is considered to be not inherently biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1985-11-18 to 1986-02-11

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: EEC-Directive 79/831, Part C: Methods for the Determination of Ecotoxicity,

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge:municipal wastewater treatment plant, F-Sindlingen

Duration of test (contact time):

ca. 29 d

Initial conc.:

ca. 58 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Test vessel: Erlenmeyer flasks 20 mL
- Test temperatur: 21 °C
- pH: 7.1
- pH adjusted: no
-Other: Stock solution: 5 g/L in distilled water

DETERMINATION OF DOC:
Apparatus: IONICS 1270M-TOC analyzer in duplicate. Evaluation after the heights of peak, averaging and expression through their own computere quipment.

Reference substance:

other: hydroquinone

Preliminary study:

Not performed

Test performance:

The validity criteria were met.

Key result

Parameter:

% degradation (DOC removal)

Value:

< 10

Sampling time:

29 d

Details on results:

After 29 days the test substance was biodegraded < 10 %.

Results with reference substance:

After 29 days, ca. 73% of the positive control was degraded.

sampling time	control DOC mg/L	test item DOC, mg/L	test item degradation %	positive control DOC mg/L	positive control DOC degradation %
3h	2.9	18.9	5.5	16.6	17
ld	2.1	18.2	9	17.7	11.6
3 d	2	19	5	17	15
7d	2	19	5	5.4	73
10 d	2.1	21.7	0	6.9	65.5
17 d	3.7	21.2	0	5.7	71.5
21 d	0.9	30**	-	5.5	72.5
24 d	1.6	22.4	0	5.8	72.5
28 d	2.6	21.4	0	5.4	71
29 d	1.9	19.1	4.5	5.4	73

** outlier

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

After 29 days of treatment, below 10% of the substance was degraded. The test substance is considered to be not ready biodegradable

Executive summary:

The ready biodegradability of the test substance was tested in the modified OECD 301E screening test. After 29 days of treatment, below 10% of the substance was degraded. The test substance is considered to be not ready biodegradable.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Modified guideline study without information on GLP

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

yes

Remarks:

see below

Principles of method if other than guideline:

Deviations from OECD 302 B: Concentration of dyestuff: 100 mg/L; concentration of activated sludge: 0.5 g/L dry material; feeding of the inoculum: each week 100 mg/L yeast extract (in most cases); test duration: up to 42 days; analytical methods: extinction at absorption maximum and DOC (dissolved organic carbon); initial DOC concentration: calculated from stock solution measurement

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Remarks:

These treatment plants received communal and/or industrial wastewater.

Details on inoculum:

- Source of inoculum/activated sludge: from communal and/or industrial waste water treatment plants.

Duration of test (contact time):

28 d

Initial conc.:

13 mg/L

Parameter followed for biodegradation estimation:

DOC removal

Reference substance:

not specified

Key result

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Details on results:

9 % adsorption after 3 h (DOC- and photometricdetermination at 580 nm)

Validity criteria fulfilled:

not specified

Interpretation of results:

not inherently biodegradable

Conclusions:

Not biodegradable, but 60% color elimination after 28 days.

Executive summary:

DOC start [mg/L]	DOC ads [%]	DOC elim [%]	DOC cat	col ads [%]	col elim [%]	col cat	elim cal	lambda [nm]	time [d]
13	9	-53	D	9	60	B	-	580	28

Description of key information

The test substance is considered to be not readily / inherently biodegradable. 

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater

Additional information

The ready biodegradability of the test substance was tested in the modified OECD 301E screening test and the inherent biodegradability in a modified Zahn-Wellens test. No biodegradation was observed in either test system.