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EC number: 287-574-8 | CAS number: 85536-87-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1990-02-08 to 1991-02-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Provenienza:Hoechst wastewater biological treatment plant
- Concentration of sludge: 10 g/L, 1020 mg/L of dry weight - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 44 other: mg C/L
- Based on:
- DOC
- Initial conc.:
- 122 other: mgO2/L
- Based on:
- COD
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD guideline.
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 22 °C
- pH: 7.3
- pH adjusted: yes with NaOH
- Aeration of dilution water: no
TEST SYSTEM
- Culturing apparatus: according to OECD guideline.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no - Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 4
- Sampling time:
- 28 d
- Details on results:
- After 29 days of treatment the substance was degraded less than 10%. (~4%)
- Results with reference substance:
- No test with reference substance was performed.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- After 29 days of treatment the substance was degraded less than 10%. (~4%). The test substance is considered to be not inherently biodegradable.
- Executive summary:
The test substance was tested by OECD 302B (Zahn-Wellens test). After 29 days of treatment the substance was degraded less than 10%. (~4%). The test substance is considered to be not inherently biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1985-11-18 to 1986-02-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: EEC-Directive 79/831, Part C: Methods for the Determination of Ecotoxicity,
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge:municipal wastewater treatment plant, F-Sindlingen
- Duration of test (contact time):
- ca. 29 d
- Initial conc.:
- ca. 58 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test vessel: Erlenmeyer flasks 20 mL
- Test temperatur: 21 °C
- pH: 7.1
- pH adjusted: no
-Other: Stock solution: 5 g/L in distilled water
DETERMINATION OF DOC:
Apparatus: IONICS 1270M-TOC analyzer in duplicate. Evaluation after the heights of peak, averaging and expression through their own computere quipment. - Reference substance:
- other: hydroquinone
- Preliminary study:
- Not performed
- Test performance:
- The validity criteria were met.
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- < 10
- Sampling time:
- 29 d
- Details on results:
- After 29 days the test substance was biodegraded < 10 %.
- Results with reference substance:
- After 29 days, ca. 73% of the positive control was degraded.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- After 29 days of treatment, below 10% of the substance was degraded. The test substance is considered to be not ready biodegradable
- Executive summary:
- The ready biodegradability of the test substance was tested in the modified OECD 301E screening test. After 29 days of treatment, below 10% of the substance was degraded. The test substance is considered to be not ready biodegradable.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Modified guideline study without information on GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- yes
- Remarks:
- see below
- Principles of method if other than guideline:
- Deviations from OECD 302 B: Concentration of dyestuff: 100 mg/L; concentration of activated sludge: 0.5 g/L dry material; feeding of the inoculum: each week 100 mg/L yeast extract (in most cases); test duration: up to 42 days; analytical methods: extinction at absorption maximum and DOC (dissolved organic carbon); initial DOC concentration: calculated from stock solution measurement
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Remarks:
- These treatment plants received communal and/or industrial wastewater.
- Details on inoculum:
- - Source of inoculum/activated sludge: from communal and/or industrial waste water treatment plants.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 13 mg/L
- Parameter followed for biodegradation estimation:
- DOC removal
- Reference substance:
- not specified
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- 9 % adsorption after 3 h (DOC- and photometricdetermination at 580 nm)
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- Not biodegradable, but 60% color elimination after 28 days.
- Executive summary:
DOC start [mg/L]
DOC ads [%]
DOC elim [%]
DOC cat
col ads [%]
col elim [%]
col cat
elim cal
lambda [nm]
time [d]
13
9
-53
D
9
60
B
-
580
28
Referenceopen allclose all
Biodegradation within 28 days
time | elimination | biodegradation | mean |
3h | 0% | 0% | < 10% |
7 days | 2% | 2% | |
14 days | -8% | -8% | |
21 days | -2% | -2% | |
28 days | 4% | 4% |
sampling time | control DOC mg/L |
test item DOC, mg/L | test item degradation % |
positive control DOC mg/L |
positive control DOC degradation % |
3h | 2.9 | 18.9 | 5.5 | 16.6 | 17 |
ld | 2.1 | 18.2 | 9 | 17.7 | 11.6 |
3 d | 2 | 19 | 5 | 17 | 15 |
7d | 2 | 19 | 5 | 5.4 | 73 |
10 d | 2.1 | 21.7 | 0 | 6.9 | 65.5 |
17 d | 3.7 | 21.2 | 0 | 5.7 | 71.5 |
21 d | 0.9 | 30** | - | 5.5 | 72.5 |
24 d | 1.6 | 22.4 | 0 | 5.8 | 72.5 |
28 d | 2.6 | 21.4 | 0 | 5.4 | 71 |
29 d | 1.9 | 19.1 | 4.5 | 5.4 | 73 |
** outlier
Description of key information
The test substance is considered to be not readily / inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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