2,4-dichloro-1-(trichloromethyl)benzene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987-07-01 to 1987-07-30

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2,4-Dichlorobenzotrichloride

IUPAC Name:

2,4-Dichlorobenzotrichloride

Constituent 2

Reference substance name:

2,4-dichloro-1-(trichloromethyl)benzene

EC Number:

235-868-1

EC Name:

2,4-dichloro-1-(trichloromethyl)benzene

Cas Number:

13014-18-1

Molecular formula:

C7H3Cl5

IUPAC Name:

2,4-dichloro-1-(trichloromethyl)benzene

Test material form:

other: solid

Details on test material:

Please refer to confidential details on test material

Method

Target gene:

In the Salmonella typhimurium strains (TA 1535, TA 100, TA 1537, TA 1538, TA 98) the amino acid histidine locus is the target gene.
In the Escherichia coli strain (WP2 uvrA) the amino acid tryptophan locus is the target gene.

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

Rat liver S-9 mix from Arochlor 1254 induced rats

Test concentrations with justification for top dose:

0, 0.16, 0.8, 4, 20, 100, 500, 2500, 5000 µg/plate

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

yes

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

sodium azide

benzo(a)pyrene

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

very toxic (thinning of bacterial lawn and reduction in the number of colonies) at doses of 100 µg/plate

Test results 2

Species / strain:

S. typhimurium TA 1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

very toxic (thinning of bacterial lawn and reduction in the number of colonies) at doses of 100 µg/plate

Test results 3

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

very toxic (thinning of bacterial lawn and reduction in the number of colonies) at doses of 100 µg/plate

Test results 4

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

very toxic (thinning of baterial lawn and reduction in the number of colonies) at doses of 100 µg/plate

Test results 5

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

very toxic (thinning of baterial lawn and reduction in the number of colonies) at doses of 100 µg/plate

Applicant's summary and conclusion

Conclusions:

2,4-Dichlorobenzotrichloride is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at dose levels up to 5000 µg/plate.