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EC number: 939-578-2 | CAS number: -
- Life Cycle description
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- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 May - 30 June 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted April 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All concentrations including the control
- Sampling method: At the start of the test (0 hour), 5 mL new media samples were taken from the freshly prepared control and test media flasks. At 24h and at the end of the test period (48 hours), 5 mL old media samples were taken from pooled control and test vessels. The samples were taken using an air displacement pipette fitted with a plastic pipette tip into 20 mL glass scintillation vials containing 5 mL of methanol.
- Sample storage conditions before analysis: no, samples were analysed on the day of receipt - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Astock solutionwas prepared by adding 37.49 – 37.53 mg of test substanceto 500 mL of autoclaved Elendt M4 medium. The test solutions were prepared by serial dilution of the stock solution.
- Other relevant information: The test media and the control were checked for the tyndall effect using a Hach 2100 N turbidity meter. The turbidity measurement for the test solution was not significantly different to the measurement for the control and therefore the test solution was considered a true solution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: cultured in 1 litre glass beakers containing 1000 mL of Elendt M4 medium. Fed daily with a concentrated suspension of Chlorella vulgaris
- Source: in-house culture setup originally from dormant eggs (ephippia) supplied by MicroBioTests Inc., Belgium
- Feeding during test: no - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 19.3 – 20.0 °C
- pH:
- 7.52 – 8.89
- Dissolved oxygen:
- ≥ 7.72 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations: 0.8, 1.8, 4.0, 8.8 and 19.4 mg a.i./L
For the individual components, recovery rates between 80 - 120 % of nominal were not maintained throughout the test as such, TWA were calculated at each concentration for each component. As the TWA concentrations were all within 80 - 120 % of nominal the EC50 values, the corresponding NOEC and LOEC values were determined based on nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: tall form beakers
- Type (delete if not applicable): covered with clear plastic petri dish lids
- Material, size: glass, 100 mL
- Volume of solution: 50 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): renewal at ca. 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: sterilised Elendt M4 medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: at 0 and 48 h (continuously measurment of temperature)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16-hour light: 8 hour dark cycle (fluorescent lighting)
EFFECT PARAMETERS MEASURED: Mobility after 24 and 48 hours
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1, 10 and 100 mg a.i./L
- Results used to determine the conditions for the definitive study: LC50 (48h). 1.0- 10.0 mg a.i./L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.17 mg/L
- 95% CI:
- >= 5.41 - <= 7.04
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- based on eight representative constituents
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No
- Mortality of control: No
- Immobilisation of control: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no precipitation observed. The test concentrations were noted to be colourless solutions during the test, with some foaming noted at the 8.8 and 19.4 mg a.i./L concentrations. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Dose-response test: yes
- 48 h-EC50: 1.48 mg/L (95% CI : 0.746 - 2.92) - Reported statistics and error estimates:
- Statistical analysis was performed using the CETIS program v 1.8.6.8.
The NOEC and LOEC values were determined using Cochran-Armitage Trend Step-Down Test at 24 and 48-hours. The 48-hour effect concentration (EC50) was determined using a Trimmed Spearman-Kärber test. The 24-hour effect concentration (EC50) was determined empirically because ≥50% immobilisation was not seen at this timepoint. - Validity criteria fulfilled:
- yes
- Remarks:
- Please refer to table 3 at "Any other information on results incl. tables"
Reference
Table 1: Time Weighted Average Concentrations
Percentage Recoveries Percentage Recovery (%) |
||||||||
Nominal Test Substance Concentration (mg a.i./L) |
Magnesium dodecyl sulfate |
Dodecan-1-ol, ethoxylated (1 EO), sulfate, magnesium salt |
Dodecan-1-ol, ethoxylated (2 EO), sulfate, magnesium salt |
Dodecan-1-ol, ethoxylated (3 EO), sulfate, magnesium salt |
Magnesium tetradecyl sulfate |
Tetradecan-1-ol, ethoxylated (1 EO), sulfate, magnesium salt |
Tetradecan-1-ol, ethoxylated (2 EO), sulfate, magnesium salt |
Tetradecan-1-ol, ethoxylated (3 EO), sulfate, magnesium salt |
0.8 |
93.0 |
89.1 |
88.8 |
88.2 |
83.4 |
82.6 |
82.3 |
83.5 |
1.8 |
113 |
106 |
101 |
101 |
96.7 |
94.9 |
96.2 |
97.0 |
4 |
113 |
104 |
102 |
102 |
96.4 |
93.1 |
91.6 |
90.7 |
8.8 |
114 |
108 |
99.1 |
106 |
103 |
97.8 |
98.8 |
98.7 |
19.4 |
104 |
97.9 |
96.3 |
97.3 |
94.7 |
92.5 |
93.4 |
98.4 |
As all time weighted averages were within 80 – 120% of nominal for all components, nominal test substance concentrations were used to calculate the toxicity concentration.
Table 2: Results of the test, 48 h values
Nominal Concentration [mg active ingredient/L] | Number of Daphnia magna exposed | Immobility at 24-hours [%] | Immobility at 48-hours [%] |
Control | 20 | 0 | 0 |
0.8 | 20 | 0 | 0 |
1.8 | 20 | 0 | 0 |
4 | 20 | 0 | 5 |
8.8 | 20 | 15 | 90 |
19.4 | 20 | 30 | 100 |
Table 3: Validity criteria for OECD 202 (2004)
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0 % |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
≥ 7.72 mg/L |
Yes |
Description of key information
EC50 (48 h): 6.17 mg a.i./L, nominal (D. magna, OECD 202)
Key value for chemical safety assessment
Additional information
The assessment is based on the data currently available. New studies, based on the category review and the final decisions issued for some of the category substances, which are also relevant for this assessment, are currently being conducted. The hazard assessment with respect to aquatic toxicity will be updated once all ongoing studies have been finalised.
The acute toxicity of Alcohols, C12-14 (even-numbered), ethoxylated, magnesium salts, < 2.5 mol EO to aquatic invertebrates was investigated in a study following OECD GD 202. The test organism Daphnia magna was exposed to nominal test concentrations of 0.8, 1.8, 4.0, 8.8 and 19.4 mg a.i./L in a semi-static test approach. The test concentrations were analytically monitored by LC-TQMS. Eight constituents of the UVCB substance were targeted in the analysis. The determined EC50 (48 h) was 6.17 mg a.i./L, based on nominal exposure concentrations.
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