[[(phosphonomethyl)imino]bis[ethylenenitrilobis(methylene)]]tetrakisphosphonic acid, ammonium salt

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Materials and methods

Principles of method if other than guideline:

Method: other: Protocol number L-58081-R019. Insufficient detail presented to assess comparability with guideline.

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

[[(phosphonomethyl)imino]bis[(ethylenenitrilo)bis(methylene)]]tetrakisphosphonic acid, sodium salt

EC Number:

244-751-4

EC Name:

[[(phosphonomethyl)imino]bis[(ethylenenitrilo)bis(methylene)]]tetrakisphosphonic acid, sodium salt

Cas Number:

22042-96-2

Molecular formula:

C9H28N3O15P5.xNa

IUPAC Name:

sodium hydrogen [10,10-dihydroxy-10-oxido-2,5,8-tris(phosphonomethyl)-2,5,8-triaza-10-phosphadec-1-yl]phosphonate

Constituent 2

Reference substance name:

[[(phosphonomethyl)imino]bis[(ethylenenitrilo)bis(methylene)]]tetrakisphos phonic acid, sodium salt

IUPAC Name:

[[(phosphonomethyl)imino]bis[(ethylenenitrilo)bis(methylene)]]tetrakisphos phonic acid, sodium salt

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Administration / exposure

Type of coverage:

not specified

Vehicle:

other: aqueous solution, administered undiluted.

Duration of exposure:

No data

Doses:

2000 mg/kg bw (equivalent to 860 mg active salt/kg bw)

No. of animals per sex per dose:

10

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

>= 2 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

>= 860 mg/kg bw

Based on:

act. ingr.

Remarks:

active salt

Remarks on result:

other: equivalent to 662 mg active acid/kg bw

Any other information on results incl. tables

Calculation of equivalent dose: Based on DTPMP-8Na (lot number NBP 269281) composed of 43% active salt including minor components, 57% water (analytical results).

For purposes of comparison with other Group 3 results, 860 mg active salt/kg bw is equivalent to 662 mg parent acid (DTPMP, CAS 15827-60-8)/kg bw.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In an acute dermal toxicity study, conducted prior to the adoption of OECD Test Guidelines and GLP, available only as a summary, the LD50 for DTPMP-8Na was ≥2000 mg/kg bw in rabbits (equivalent to 860 mg active salt/kg bw and 662 mg active acid/kg bw).