Registration Dossier
Registration Dossier
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EC number: 226-166-6 | CAS number: 5308-25-8
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- no data on test substance purity
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute Toxicologic Evaluation of 1-Ethylpiperazine
- Author:
- Bomhard E., Pauluhn J., Ruf J.
- Year:
- 1 997
- Bibliographic source:
- International Journal of Toxicology, 16(2), 60 - 61
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1-ethylpiperazine
- EC Number:
- 226-166-6
- EC Name:
- 1-ethylpiperazine
- Cas Number:
- 5308-25-8
- Molecular formula:
- C6H14N2
- IUPAC Name:
- 1-ethylpiperazine
- Details on test material:
- - Name of test material (as cited in study report): 1 (N)-Ethylpiperazine
- Analytical purity: no data
No further information available.
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 3.1 - 3.4 kg
No further information.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 µL - Duration of treatment / exposure:
- 24 h
- Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): rinsed with physiologic saline
- Time after start of exposure: 24 h
SCORING SYSTEM: according to Draize; the irritation indices were calculated in accordance with Guideline 83/467/EC No. 8
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not specified
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- not specified
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not specified
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- other: 24 - 48 - 72 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not specified
- Irritant / corrosive response data:
- After 7 d the nictitating membrane and the palpebral conjunctiva showed necrotic changes. Therefore, the test substance was found to cause severe damage to the eyes.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
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