Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

German standard guideline DIN 38412 part 27, WoE, validity 4:
30min-EC50 (Pseudomonas putida) = 800 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
800 mg/L

Additional information

A weight of evidence approach was used with two studies on the test substance γ-decalactone.

The first study, according to German standard guideline DIN 38412 part 27, was performed to assess the toxicity of γ-decalactone on microorganisms Pseudomonas putida. Five test concentrations were prepared with two controls (control and solvent control) and oxygen consumption was recorded after 30 min of exposure. According to the little information, the 30min-EC50 was evaluated to be 800 mg/L. No sufficient information was provided in this report to assess the fiability of the results.

The second study, not according to international or national guideline, was performed to assess the toxicity of gamma-decalactone on the respiration of activated sludge. A limit test was performed at 1000.5 mg/L with activated sludge from a wastewater treatment plant laboratory with municipal wastewater. According to the little information provided in this report, no significant effect is determined at the concentration tested. The respiration rates of blank and test substance were 20 and 26 mg O2/(L*h), respectively. The 30-min EC50 was estimated to be greater than the highest concentration tested of 1000.5 mg/L. No sufficient information was provided in this report to assess the fiability of the results.

Based on the first study, 30min-EC50 was determined to be 800 mg/L for Pseudomonos putida. This result was used as key value for chemical safety assessment.