2-(2-heptadec-8-enyl-2-imidazolin-1-yl)ethanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

other: OECD Series on Testing and Assessment No. 23

GLP compliance:

yes

Specific details on test material used for the study:

- Physical state: yellow to brown, clear liquid
- Analytical purity: 96.6 %
- Batch No.: 05774HP8
- Storage condition of test material: The test substance was stored at ambient temperature

Analytical monitoring:

yes

Details on sampling:

Samples for concentration control analysis were taken from each freshly prepared solution (0 h and 24 h) from additionally prepared test vessels without daphnids and at the end of each exposure interval (after approx. 24 h and 48 h) from all test vessels with mobile daphnids and the lowest concentration with 100% immobilisation.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The stock test solution (1 mg/L) was prepared by directly adding 2 mg of test substance to 2 L of test medium and stirring for approximately 1 day at 20 ± 2 °C. The lower test concentrations were prepared by dilution of this stock solution.
Before the dilution was performed, the stock solution was visually checked for complete dissolution of the test substance. The solution was further inspected for the presence of any undissolved test substance by observing the scatting of a laser light through the test solution (i.e. Tyndall effect). The stock solution was visibly colorless and clear and no scattering of laser light was observable. The test vessels were saturated with test solution for approx 1 day before the start of exposure. Fresh stock and test solutions were prepared daily.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation: < 24 hours (at least 3rd brood progeny)
- Feeding during test: no

CULTURE CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Culture media: synthetic fresh water (Elendt M4) prepared according to OECD 202
- Temperature: 20 ± 1 °C
- Diet: unicellular algae Desmodesmus subspicatus
- Feeding: Daily
- Renewal of the culture water: Twice weekly

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.48 mmol/L

Test temperature:

19.4 - 19.8 °C

pH:

7.7 - 7.8

Dissolved oxygen:

8.5 - 8.9 mg/L

Nominal and measured concentrations:

- Nominal test concentrations: 0 (control), 0.046, 0.10, 0.22, 0.46, 1.00 mg/L (based on range-finding study)
- Mean measured test concentrations: --, 0.027, 0.064, 0.144, 0.326, 0.837 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: glass beakers, 50 ml, 50 mL
- Aeration: none
- Renewal rate of test solution: 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.1 animals/mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water (Elendt M4) is used as media for culture and test purposes.
- Alkalinity: 0.90 mmol/L
- Ca/Mg ratio: about 4 : 1
- Conductivity: 588 μS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and oxygen content of the test solutions was measured after 0 h and 24 h (new test solutions) and after 24 h and 48 h (old test solutions) in replicate 1 of each tested concentration. Temperature was measured continuously.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: about 168 – 594 lux at a wave length of 400 -750 nm

EFFECT PARAMETERS MEASURED: immobilization and abnormal behaviour and appearance after 0, 24 and 48 hours

TEST CONCENTRATIONS
- Range finding study: yes
- Results used to determine the conditions for the definitive study: the 48 h EC50 was between 0.1 and 1.0 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.163 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.064 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.326 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: none
- Dissolution behaviour: no remarkable observations, clear test medium

Results with reference substance (positive control):

The EC50 (24h) of the reference substance potassium dichromate was 0.89 mg/L. Date of the last control experiment: 13 Apr 2010.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

study without analytical monitoring

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): (TK 10648)
- Physical state: liquid
- Analytical purity: comm. grade
- Batch No.: EN 46033.52
- Storage condition of test material: room temperature

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 0.1 g test substance was dissolved in and made up to 10 mL with DMF. This solution was diluted to 10 mg/L with water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Ciba-geigy Ltd., Facilities, Basle
- Age at study initiation: ≤ 24 h
- Feeding during test: none

BREEDING
- Culture vessel: glass vessels containing approx. 2.5 L of reconstituted water
- Temperature: 20 ± 1 °C
- Water renewal: partially thrice weekly
- Type and amount of food: suspension of green algae (Scenedesmus subspicatus) supplemented by a suspesnion of Tetramin-extract in such quantities that the food is consumed after 24 h

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Hardness:

240 mg/L (as CaCO3)

Test temperature:

20 ± 1 °C

pH:

7.6 - 7.9

Dissolved oxygen:

90 - 95%

Nominal and measured concentrations:

- Nominal test concentrations: 0 (control), 0.10, 0.18, 0.32, 0.58, 1.0 mg/L
- No analytical verification of test concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers covered with watch glasses 100 mL solution per beaker
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstitited water, aerated with clean air for at least 24 h before use
- Intervals of water quality measurement: oxygen, pH, temperature daily (in separate vessels at start of the test)

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light
- Light intensity: approx. 200 Lux

EFFECT PARAMETERS MEASURED: mobility after 24 hours

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.37 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 0.30 - 0.46 mg/L

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

< 0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Immobilization in blank: 0%
- Immobilization in vehicle: 0%

Description of key information

The substance is acutely very toxic to aquatic invertebrates. The 48-h EC50 value is 0.163 mg/L in aquatic invertebrates (Daphnia magna).

Additional information

The acute toxicity to aquatic invertebrates was determined in a study according to OECD TG 202 and OECD No. 23 (Aquatic toxicity testing of difficult substances and mixtures) that was in compliance with GLP criteria (BASF, 2010). In this study, daphnids (D. magna, 20 per concentration) were exposed to nominal concentrations of 0 (control), 0.046, 0.10, 0.22, 0.46 and 1.00 mg/L (test range based on range-finding study) for 48 hours under semi-static conditions (test medium renewal each 24 hours). Test concentrations were analytically verified; effect values are expressed as measured. No immobility was observed in the control. The 48-h EC50 value was determined at 0.163 mg/L (measured).

As supporting data a study is available that determined the toxicity to daphnids (Daphnia magna) according to OECD 202, where DMF was used as vehicle (BASF, 1988). Test organisms were exposed to nominal test concentrations of 0 (control), 0.10, 0.18, 0.32, 0.58, 1.0 mg/L for 24 hours. Test concentrations were not analytically verified. No immobility was observed in the control (blank) and vehicle control. Regarding the test substance, the 24-h EC50 was determined at 0.37 mg/L.