Registration Dossier
Registration Dossier
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EC number: 200-453-6 | CAS number: 60-09-3
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: only abstract available
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- 4-Aminoazobenzene
- Author:
- Beratergremium für Altstoffe (BUA)
- Year:
- 1 998
- Bibliographic source:
- BUA-Stoffbericht 217. BUA der Gesellschaft dt. Chemiker, S. Hirzel Wissenschaftliche Verlagsgesellschaft, Stuttgart
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Reference Type:
- secondary source
- Title:
- IUCLID
- Author:
- ECB
- Year:
- 2 007
- Bibliographic source:
- ECB IUCLID Dataset
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 4-aminoazobenzene
- EC Number:
- 200-453-6
- EC Name:
- 4-aminoazobenzene
- Cas Number:
- 60-09-3
- Molecular formula:
- C12H11N3
- IUPAC Name:
- 4-[(E)-2-phenyldiazen-1-yl]aniline
- Details on test material:
- - Name of test material (as cited in study report): 4-Aminoazobenzene
- Analytical purity: 98.7%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- no data
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- head only
- Vehicle:
- other: acetone/polyethyleneglycol (1:1)
- Details on inhalation exposure:
- TEST ATMOSPHERE (if not tabulated)
- MMAD (Mass median aerodynamic diameter): 62 - 93% of the aerosol particles had a MMAD of ≤ 3µm
- Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 0, 0.023, 0.341, 2.80 mg/l analytical concentration
- No. of animals per sex per dose:
- 5 female and 5 male animals per dose
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: no data
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 2.8 mg/L air
- Exp. duration:
- 4 h
- Mortality:
- no mortality occured
- Clinical signs:
- other: at mid and high dose bradypnoe, cyanosis; reduced body temperature at mid and high dose; dose dependent increase in methemoglobin concentration (marginal at low dose); no symptoma of intoxication reported on the 4th day after exposure
- Body weight:
- reduced body weight gain at high dose
- Gross pathology:
- no treatment related effects observed at macroscopical pathology
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
