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EC number: 263-157-6 | CAS number: 61790-66-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From May 6, 1997 to May 10, 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- No analytical dose verification
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- ; no details on validity criteria is available. No analysis of the test solutions was peformed; but this deviation can be considered acceptable as the test substance is water soluble.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: 2 gm of the test substance was dispersed in reverse osmosis water using ultrasonification. The volume was adjusted to 1 L to give 2 gm/L stock concentration. A serial dilution was done to prepare a 200 mg/L stock. Aliquots of (10 and 30 mL) from 200 mg/L stock disepersion and aliquots (10, 30, 100 and 300 mL) from 2 gm/L stock were separately dispersed in dechlorinated tap water and the volume was finally adjusted to 2 L to give 0.1, 0.3, 1.0, 3.0, 10.0, 30.0 mg/l concentrations, respectively. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Strain: Oncorhynchus mykiss
- Source: Parkwood trout farm, Harrietsham, Kent, UK
- Length at study initiation (length definition, mean, range and SD): 4.2 ± 0.2 cm
- Weight at study initiation (mean and range, SD): 1.0 ± 0.14 g
ACCLIMATION
- Acclimation period: 7d
- Health during acclimation (any mortality observed): Approximately 4% mortality
- Medication: None - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 100 mg/L CaCO3
- Test temperature:
- 14 ± 0.1°C
- Nominal and measured concentrations:
- - Nominal: 0.10, 0.30, 1.0, 3.0, 10.0 and 30.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 20 L vessels
- Aeration: Yes
- No. of organisms per vessel: 10
- Renewal rate of test solution (frequency/flow rate): None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water was dechlorinated by passage through an activated carbon filter (Purit series 500) and partly softened (Elga Nimbus 1248D duplex water softener). Water was then passed through a series of computer controlled plate heat exchangers to achieve the required temperature
- Hardness: 100 mg/L CaCO3
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality rates, swimming at the bottom and surface of test vessel, loss of equilibrium and presence of moribund fish were observed at 3, 6, 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Test concentrations: Nominal: 0.10, 0.30, 1.0, 3.0, 10.0, 30.0 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 1.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: (95% CI: 0.84-1.7 mg/L)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- ca. 3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Sub-lethal effects: No abnormalities observed up to the concentration of 0.30 mg/L. Swimming at the bottom and surface of test vessel, loss of equilibrium, presence of moribund fish and mortality were observed at the concentrations >1.0 mg/L at 3, 6, 24, 48, 72 and 96 h.
- Mortality of control: No
- Effect concentrations exceeding solubility of substance in test medium: The test media at the concentrations of 0.10, 0.30, 1.0 and 3.0 mg/L were clear colorless solutions and the test media at the concentrations of 10 and 30 mg/L were opaque dispersions. - Sublethal observations / clinical signs:
Mortality was determined based on moving average method by Thompson, 1974.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the study conditions, analysis of the mortality data by the moving average method of Thompson (1947) yielded a nominal 96 h LC50 value of 1.2 mg/L. The nominal 96 h NOEC and LC100 were determined at 0.3 and 3.0 mg/L, respectively.
- Executive summary:
A study was conducted to assess the acute toxicity of the test substance, C16-18 and C18-unsatd. DEA, to fish (Rainbow trout) according to the OECD Guideline 203. Ten fish per test vessel were exposed to the test substance (dispersed in water using ultrasonication) at the concentrations of 0.1, 0.3, 1.0, 3.0, 10.0 and 30.0 mg/L for 96 h under static conditions. Mortality and other sub-lethal effects such swimming at the bottom and surface, loss of equilibrium and morbidity were recorded at 3, 6, 24, 48, 72 and 96 h. No analytical dose verification was conducted. Under the study conditions, analysis of the mortality data by the moving average method of Thompson (1947) yielded a nominal 96 h LC50 value of 1.2 mg/L. The nominal 96 h NOEC and LC100 were determined at 0.3 and 3.0 mg/L, respectively (Wetton 1997).
Reference
Description of key information
A study was conducted to assess the acute toxicity of the test substance, C16-18 and C18-unsatd. DEA,to fish (Rainbow trout) according to the OECD Guideline 203. Ten fish per test vessel were exposed to the test substance (dispersed in water using ultrasonication) at the concentrations of 0.1, 0.3, 1.0, 3.0, 10.0 and 30.0 mg/L for 96 h under static conditions. Mortality and other sub-lethal effects such swimming at the bottom and surface, loss of equilibrium and morbidity were recorded at 3, 6, 24, 48, 72 and 96 h. No analytical dose verification was conducted. Under the study conditions, analysis of the mortality data by the moving average method of Thompson (1947) yielded a nominal 96 h LC50 value of 1.2 mg/L. The nominal 96 h NOEC and LC100 were determined at 0.3 and 3.0 mg/L, respectively (Wetton 1997).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 1.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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