Sodium polysulfide aluminosilicate with a SOD-type framework structure

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

OECD guideline 203. GLP study.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

The Notice on the Test Method Concerning New Chemical Substances < Algal Growth Inhibition Test, Daphnia Acute Immobilization Test, and Fish Acute Toxicity Test >

GLP compliance:

yes

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Solubility and stability of the test substance in the test medium: Since the test substance is a poorly water-soluble inorganic compound, the test was carried out using the test item in the dispersed state. The ratio of the measured value to the nominal value was 91 to 92% at the time of preparation of the test solution and 89 to 90% after 24 hours (renewal). As a reference, the filtrate obtained by filtering the test solution (0.45μm) was similarly analyzed to confirm the concentration of the test substance dissolved in the test solution; the average value of the test substance concentration in the filtrate was less than the detection limit (<0.296 mg/L).

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 (control), 100 mg/L
- Sampling method: the concentration of test item was verified at 0 and 24 hours.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Since the test substance is a poorly water-soluble inorganic compound, the test was carried out using the test item in the dispersed state.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Japanese rice fish (medaka)
- Strain: Latipes

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

24 ± 1ºC

Nominal and measured concentrations:

- 0 (control), 100 mg/L (nominal); 90.2 mg/L (measured).
- The ratio of the measured value to the nominal value was 91 to 92% at the time of preparation of the test solution and 89 to 90% after 24 hours.

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 5.0 L/vessel
- Renewal rate of test solution (frequency/flow rate): 24h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: room light (less than 1000 lux)

EFFECT PARAMETERS MEASURED: mortality

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 90.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 90.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 90.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: none reported
- Dissolved oxygen concentration: not specified

Validity criteria fulfilled:

yes

Conclusions:

The 96h-LC50 of the test item in rice fish was determined to be > 90.2 mg/L, based on measured concentration.

Executive summary:

An acute fish toxicity test was conducted according to OECD 203, under GLP conditions. To establish the toxicity of the test material to the rice fish (Oryzias latipes), a limit test was performed under semi-static conditions (renewal rate 24h). 10 fish per concentration were exposed to 100 mg/L test item (nominal concentration) or the control for 96 h at 24 ± 1 ºC. No mortality was observed. The concentration of test item was verified at 0 and 24 hours by ICP-MS. Under test conditions, the 96h-LC50 of the test item in rice fish was determined to be > 90.2 mg/L, based on measured concentration.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

June 1976

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

The study is not well documented. No GLP study.

Qualifier:

no guideline followed

Principles of method if other than guideline:

No data on test guideline

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Number of test organisms per vessel: 10
Average weight: 1.5g
Average length: 49.8 mm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

36 (CaCO3)

Test temperature:

15ºC. Range during test 14.5 to 15.5ºC

pH:

Initial pH: 7.85. Range during test: 7.4 to 7.8

Dissolved oxygen:

91% saturation, range during test 84 to 98% saturation

Details on test conditions:

Test vessel material: glass
Test vessel capacity: 5 litres
Dimensions of the vessel: 160mm(W)x300 mm(H)

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

other: TLm=TL50

Effect conc.:

> 32 000 mg/L

Conc. based on:

test mat.

Remarks on result:

other: TLm=TL50 Median Lethal Time

Validity criteria fulfilled:

not specified

Conclusions:

The result was as follows:
96hr TLm (Median Lethal Time, or TL50) >32000 mg/l

Executive summary:

The test was conducted to establish the toxicity of the test material to the Rainbow Trout fishes.

There is no information about the used guideline.

There is no information about the tested concentration.

The result was as follows:

96hr TLm (Median Lethal Time, or TL50) >32000 mg/l

Description of key information

Key study. Method according to OECD 203, GLP study. The 96h-LC50 of the test item in rice fish (Oryzias latipes) was determined to be > 90.2 mg/L (measured concentration).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

90 mg/L

Additional information

Supporting study. Data from a supporting study reports a 96-hr TLm > 32000 mg/L in Rainbow Trout (Oncorhynchus mykiss). No further information is available.