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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Link to relevant study record(s)

Description of key information

Two toxicity studies on earthworms are available and included as supporting information. More details are provided in the additional information.


Key value for chemical safety assessment

Additional information

Studies required in Annex VII and some other studies required for higher tonnage are available. All the studies available have been included in the dossier for this endpoint. There are two studies available for this endpoint, one short term and one long-term study on earthworms. Both studies were performed according to standard guidelines and under GLP. 

Short term: Eisenia fetida (> 2 months old with clitellum; group mean wet mass of 196 - 253 mg) were exposed for 14 days to nominal concentrations of 62.5, 125, 250, 500 and 1000 mg/kg soil dw and a blank control. The test organisms were not fed during the test. The test conditions were: temperature 20 ± 1 °C, pH 6.8 - 7.2, moisture content 33 - 41% of soil dw, light continuous, 400 - 800 Lux. Assessments of mortality and flaccidity were made on day 7 and 14. Average live weight was recorded at day 0 and day 14. The 14-d LC50 was determined to be > 1000 mg/kg soil dw, based on nominal concentrations (Rufli 1989).

Long-term: The toxicity of the test substance formulation (A6943E WG20) to adult Eisenia fetida (about 3 months old with clitellum; weight 261 – 466 mg) was studied by exposing them for 8 weeks to nominal concentrations corresponding to 62.5, 125, 250, 500 and 1000 mg/kg (12.7, 25.4, 50.75, 101.5, 203 mg triasulfuron/kg) dw soil and to a blank control. After four weeks, the adult worms were removed. The juvenile worms remained in the test vessels for a further 4 weeks. The test conditions were maintained at 18.0 - 22.0 ˚C; 16-hour light and 8-hour darkness (light intensity: 720 lux), pH 5.95 – 6.27, the water content of soil 55.9 -56.4% of WHC. The 4-week NOEC for biomass and reproduction was determined to be 250 and 500 mg/kg (101.5 and 203 mg triasulfuron/kg) soil d.w., respectively, based on nominal concentrations (Friedrich 2010).