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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

EC50 >= 0.19 µg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
0.19 µg/L

Additional information

The acute toxicity of the test item to Daphnia magna was determined in a 48-hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 (1992) and the OECD Guideline No. 202 (2004).
A limit test was performed to demonstrate that the test item has no toxic effect on the test organisms up to its solubility limit in test water. 
Due to the low water solubility of the test item, a dispersion of the test item with the loading rate of 100 mg/L was continuously stirred over 3 hours. Then, the dispersion was filtered through a 0.45 µg membrane filter. The undiluted filtrate was used as test medium. Additionally, a control was tested in parallel. The analytically measured test item concentration in the analyzed test medium samples (the undiluted filtrate of the supersaturated dispersion) was 0.19 µg/L at the start of the test. In this test medium, incubated under the conditions of the test (but without daphnia), the test item concentration slightly decreased to 0.16 µg/L during the test period. The mean measured test item concentration was calculated to be 0.17 µg/L (geometric mean over the measurements at the start and the end of the test).
The biological results were related to the loading rate of 100 mg/L (mean measured 0.17 µg/L).
In the control and at the loading rate of 100 mg/L, no immobilized test organisms were observed during the test period of 48 hours. 
Therefore, the 48-hour NOEC and the 48-hour EC0 of the test item to Daphnia magna were determined to be the loading rate of 100 mg/L. The 48-hour NOEC and the 48-hour EC0 might even be higher, concentrations above the solubility limit of the test item in the test water at the loading rate of 100 mg/L were not tested in accordance with the test guideline. The 48-hour EC50 and the 48-hour EC100 were clearly higher than than the loading rate of 100 mg/L. These values could not be quantified, since the test item had no toxic effect on the daphnids up to the highest test item concentration which could be dissolved in the test water.
In conclusion, the test item had no acute toxic effects on Daphnia magna up to its solubility limit in test water under the present test conditions. The test medium was a clear solution throughout the whole test duration.