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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
05 Aug - 07 Aug, 1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1984
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Remarks:
Niedersächsisches Umweltministerium, delivered on 07 Jul, 1997
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
The stock solutions were prepared with dilution water.
The test substance has been dissolved homogeneously at all test concentrations.
Test organisms (species):
Daphnia magna
Details on test organisms:
2 to 24 h old daphnids were used for the range finding and main study. They had been obtained by removing the mother animals twice within 22 h. The daphnids were acclimated to the dilution water 2 hours before exposure.
Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
48 h
Nominal and measured concentrations:
Nominal: 1.0 - 3.2-10 - 32- 100 - 270 mg/L
Measured ranged from 97% to 104%
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
26 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: recalculated value

Please refer to "overall remark/ attached background material" field for result tables.

Table 1: Validity criteria for OECD 202 (2004)

Criterion from the guideline

Outcome

Validity criterion fulfilled

In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.

0% immobilization at the end

yes

The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.

Lowest value 8.0 mg /L

yes

Validity criteria fulfilled:
yes
Remarks:
For further details please refer to “Any other information on results incl. tables”.
Conclusions:
The present guideline study was conducted in compliance with GLP. Under the test conditions used, the EC50 48h for Daphnia magna was 26 a.s. mg/L.

Description of key information

From short-term key study, the 48-hour EC50 for aquatic invertebrates exposed to the present test material was 0.029 mg a.s./L in freshwater.

From short-term supporting study, the 96-hour LC50 for aquatic invertebrates exposed to the present test material was 0.053 mg a.s./L in saltwater.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Remarks:
This endpoint was re-calculated
Effect concentration:
ca. 0.029 mg/L

Marine water invertebrates

Marine water invertebrates
Dose descriptor:
LC50
Remarks:
supportive study
Effect concentration:
0.053 mg/L

Additional information

Four GLP-studies are available for short-term toxicity testing to aquatic invertebrates and one of them was identified as key study.

In the key study (2000), the acute toxicity of test substance to invertebrates was investigated in a 48-hours static test with Chironomus riparius according to guideline OPPTS 850.1790. Five first instar per replicate and four replicate per concentration were exposed for 48 hours under static conditions to nominal concentrations of 0.007, 0.013, 0.025, 0.050 and 0.10 mg/L, alongside with a control. Based on this study, the EC50 for aquatic invertebrates exposed to test substance in the freshwater was 0.029 mg as/L.

There are four additional studies available, one conducted with Americamysis bahia two with Daphnia magna and one Crassostrea virginica; the resulting EC50 and LC50 were 0.053 mg a.s./L, 26 a.s. mg/L, > 119 mg a.s./L and > 129.14, respectively. These studies were considered suitable for supporting purpose.

 

In the first supporting study (2000) the acute toxicity of test material to A. bahia was determined in a 96-h flow-through test according to guideline OPPTS 8501035. The 96-hour LC50 of the technical active ingredient was determined to be 0.053 mg a.s./L based on the measured concentrations.

In the second supporting study (2000) the acute toxicity of test material to daphnia was determined in a 48-h-static test according to guideline OECD 202. The 48-hour EC50 of the technical active ingredient was determined to be 26 mg a.s./L based on the nominal concentrations.

 

In the third supporting study (2000), the acute toxicity of test material to daphnia was determined in a 48-h-static test according to guideline OECD 202.The 48-hour EC50 of the technical active ingredient was determined to be > 119 mg a.s./L based on the mean measured concentrations.

 

In the fourth supporting study (1999), the acute toxicity of test material to C. virginica was determined in a 96-h flow-through limit test according to US EPA-72.3 guideline.The 96-hour EC50 of the technical active ingredient was determined to be > 129.14 mg a.s./L, based on the mean measured concentration. Based on this study, the EC50 for C. virginica exposed to test substance in the saltwater was > 129.14 mg a.s./L.